| 猪肝脏组织表达序列标签(ESTs)的初步分析 |
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| 引用本文： | 李宁,赵志辉,刘兆良,赵兴波,连正兴,吴常信.猪肝脏组织表达序列标签(ESTs)的初步分析[J].中国农业科学,2002,35(12):1525-1528. |
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| 作者姓名： | 李宁 赵志辉 刘兆良 赵兴波 连正兴 吴常信 |
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| 作者单位： | 1. 中国农业大学农业生物技术国家实验室,100094
2. 中国农业大学动物科学技术学院,100094 |
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| 基金项目： | 国家重点基础研究发展规划资助项目 (G2 0 0 0 0 161) |
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| 摘 要： | 构建了猪肝脏组织cDNA文库 ,并对文库中随机挑选的 4 38个克隆的表达序列标签 (ESTs)进行了研究。结果表明 ,在所研究的 4 38个ESTs中 ,186个在猪种中已有匹配序列 ,37个在人类及其它物种中可以找到同源序列 ,2 15个为未知功能基因。试验还测定了文库中 4 5个cDNA克隆的全长插入序列 ,结果表明 ,19个为已知功能基因 ,11个没有发现开放阅读框 ,其它 15个具有开放阅读框 ,但基因的功能未知。这些结果初步代表了肝脏组织的功能基因表达谱。
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| 关 键 词： | 猪 肝脏 cDNA文库 表达序列标签 |
| 修稿时间： | 2001年7月11日 |
Analysis of Expressed Sequence Tags from Porcine Liver Organ |
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| LI Ning ,ZHAO Zhi hui ,LIU Zhao liang ,ZHAO Xing bo ,LIAN Zheng xing ,WU Chang xin.Analysis of Expressed Sequence Tags from Porcine Liver Organ[J].Scientia Agricultura Sinica,2002,35(12):1525-1528. |
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| Authors: | LI Ning ZHAO Zhi hui LIU Zhao liang ZHAO Xing bo LIAN Zheng xing WU Chang xin |
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| Institution: | LI Ning 1,ZHAO Zhi hui 1,LIU Zhao liang 1,ZHAO Xing bo 2,LIAN Zheng xing 2,WU Chang xin 2 |
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| Abstract: | In order to study the expression of functional gene and its effect on metabolic control and other physiological functions in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species, while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size and the results showed that 19 of them were function known genes, 11 had no open reading frame ( ORF ) and 15 had ORF but the function were not elucidated yet. |
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| Keywords: | Swine Liver cDNA library Expressed sequence tags |
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