| 软体动物牡蛎肌联蛋白一级结构的预测 |
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| 引用本文： | 软体动物牡蛎肌联蛋白一级结构的预测.软体动物牡蛎肌联蛋白一级结构的预测[J].畜牧与饲料科学,2016,37(12):5-5. |
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| 作者姓名： | 软体动物牡蛎肌联蛋白一级结构的预测 |
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| 作者单位： | 内蒙古医科大学基础医学院,内蒙古呼和浩特010110 |
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| 基金项目： | 内蒙古自然科学基金项目（2014MS08106） |
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| 摘 要： | 旨在预测牡蛎肌联蛋白的基因exon-intron结构,并确定其一级结构及结构域。利用生物信息学方法,从牡蛎基因组数据中预测肌联蛋白基因的exon-intron结构,并进一步预测编码肌联蛋白的m RNA组成,从而确定该蛋白的一级结构,并检测其结构域;与同源蛋白进行序列比对,制作系统进化树以了解其在系统进化中的位置。结果表明,该蛋白基因在基因组中横跨200 kb的序列区域,预测编码肌联蛋白的mRNA大约由74 kb组成,推测相对分子质量约为2 700 ku;该蛋白由Ig、Fn、kinase结构域,PEVK结构域和unique序列组成;在系统发育方面,该蛋白归属于原口动物集团。牡蛎肌联蛋白是一种新型的肌联蛋白样蛋白。
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Prediction of Primary Structure of Oyster Connectin |
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| Abstract: | The aim of the present study was to predict the exon-intron structure of gene coding connectin in oyster and to determine its primary structure and domains. The exon-intron structure of gene coding connectin was deduced based on oyster genomic data by using bioinformatics method; the mRNA composition of coding regions for connectin in oyster was predicted; the primary structure of oyster connectin was determined and its domains were detected; phylogenetic tree was constructed by comparing amino acid sequences of homologous protein associated with connectin from different species. The results showed that the gene coding connectin in oyster genome covered 200 kb, the connectincoding mRNA was about 74 kb, and the relative molecular weight of oyster connectin was deduced as 2 700 ku; oyster connectin was composed of Ig, Fn, and kinase domain, PEVK domain and unique sequences; the oyster eonnectin phylogenetically belonged to protostome group. Oyster connectin is a new type of connectin-like protein. |
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