Assessment for salinity tolerance through intergeneric hybridisation: Triticum durum × Aegilops speltoides |
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| Authors: | Seyed Ahmad Sadat Noori |
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| Institution: | (1) Department of Agronomy and Plant Breeding, University of Tehran, Abouraihan Campus, P.O. Box 4117, Tehran, Iran |
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| Abstract: | Summary Triploid hybrid embryos (AABB × SS = ABS) were produced by crossing two moderately salt-tolerant wheats, namely Triticum turgidum durum and Aegilops speltoides subsp. speltoides. The embryos were rescued by culturing on agar No. 1. Chromosome doubling was achieved by using 0.05% colchicine. The resulting
hexaploid plants grew to maturity, and produced a considerable amount of seed (≈40,000). The synthetic hexaploid (C2) was tested for salinity tolerance, grown at 0, 100, 125, 150, 160, 170, 180 and 200 mM NaCl in a standard nutrient solution
for 2 weeks. The criterion of salt tolerance was root growth in different NaCl concentrations. The amphidiploid material showed
greater salinity tolerance than either parent, suggesting the presence of different genes for tolerance in the parents. |
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| Keywords: | amphidiploid embryo rescue salt tolerance wheat |
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