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| RAPD分子标记技术用于实验兔品系鉴定的初探 | |
| 引用本文： | 赵立虎,谢夏阳,赵丹凤,张春燕,狄敏,刘丽均,李晟阳,张守纯,徐平.RAPD分子标记技术用于实验兔品系鉴定的初探[J].上海交通大学学报(农业科学版),2007,25(1):66-70. |
| 作者姓名： | 赵立虎 谢夏阳 赵丹凤 张春燕 狄敏 刘丽均 李晟阳 张守纯 徐平 |
| 作者单位： | 1. 中国科学院,上海实验动物中心,上海,201615;沈阳农业大学,畜牧兽医学院,沈阳,110161 2. 中国科学院,上海实验动物中心,上海,201615 3. 中国科学院,上海实验动物中心,上海,201615;南京农业大学,动物医学院,南京,210095 4. 中国科学院,上海实验动物中心,上海,201615;扬州大学,比较医学中心,扬州,225009 5. 沈阳农业大学,畜牧兽医学院,沈阳,110161 |
| 基金项目： | 国家科技自然资源条件平台工作项目 |
| 摘 要： | 本文选用新西兰白兔(New Zealand White Rabbit,NZWR)、青紫兰兔(Chinchilla Rabbit,CR)、日本白兔(Japanese White Rabbit,JWR)3个品系的血样提取基因组DNA,并应用随机引物扩增多态性DNA技术(Random Amplified Polymorphic DNA,RAPD)进行遗传分析和比较。结果表明,使用随机引物PCR扩增后,扩增产物经琼脂糖凝胶电泳检测共发现78个条带,其中多态性片段25条,占32.1%,从7套共140个随机引物中筛选出10个多态性较高的引物,包括BH9、AD1、BH11、AN12、AN14、O19、AD3、AD19、O4和O14。其中BH9、AD1扩增效果最好,扩增产物有较大的共性和较好的多态性,可作为实验兔品系鉴定的分子依据。日本白兔和新西兰兔片段共享度最高为0.8268,青紫兰兔和日本白兔最低为0.6190,统计结果显示RAPD标记多态性丰富,3品系的种内相似度大于种间相似度。该技术可用于实验兔的遗传检测。 |
| 关 键 词： | 实验兔 遗传检测 |
| 文章编号： | 1671-9964（2007）01-0066-05 |
| 收稿时间： | 2006-09-28 |
| 修稿时间： | 2006年9月28日 |
RAPD Analysis of the Genetic Specificity in Laboratory Rabbits |
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| ZHAO Li-hu,XIE Xia-yang,ZHAO Dan-feng,ZHANG Chun-yan,DI Min,LIU Li-jun,Li Sheng-yang,ZHANG Shou-chun,XU Ping.RAPD Analysis of the Genetic Specificity in Laboratory Rabbits[J].Journal of Shanghai Jiaotong University (Agricultural Science),2007,25(1):66-70. | |
| Authors: | ZHAO Li-hu XIE Xia-yang ZHAO Dan-feng ZHANG Chun-yan DI Min LIU Li-jun Li Sheng-yang ZHANG Shou-chun XU Ping |
| Institution: | 1. Shanghai Laboratory Animal Center, Academia Sinica, Shanghai 201615, China; 2.College of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang 110161, China; 3.College of Veterinary, Nanjing Agricultural University, Nanjing 210095, China; 4. Comparative Medicine Center of Yangzhou University, Yangzhou 225009, Chin |
| Abstract: | Rabbit genomic DNAs derived from Japanese White Rabbits (JWR), Chinchilla Rabbits (CR) and New Zealand White Rabbits (NZWR) were analyzed with random amplified polymorphic DNA (RAPD) technique and the genetic variation among 3 rabbit strains were studied. The results showed that after 140 random primers in 7 sets were used to amplify the rabbit genomic DNA with PCR methods, 10 have been screened out on the preliminary experiments, including BH9,AD1,BH11,AN12,AN14,O19,AD3,AD19,O4 and O14. Two primers (AD1 and BH9) among the 10 are superior to others and could not only amplify the common DNA bands of 3 rabbit strain lines but also the different characteristic bands of each strain. 78 amplified fragments were detected by electrophoresis on agarose gel. Among them, 25 fragments were polymorphic, accounting for 32.1 %. It was showed that the RAPD polymorphisms among rabbit lines were abundant. The genetic similarity between JW rabbits and NZW rabbits was highest (0.8286) among 3 rabbit strains and the lowest genetic similarity between CR and JW is 0.6190. The amplified band patterns also showed that the genetic similarity inner a strain is bigger than among different strains. The RAPD technique could be used as a method to monitor genetic relationship among different rabbit strains. |
| Keywords: | RAPD |
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