| 杜梨子叶离体再生体系的建立 |
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| 引用本文： | 林静.杜梨子叶离体再生体系的建立[J].中国农学通报,2015,31(19):41-47. |
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| 作者姓名： | 林静 |
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| 作者单位： | 新疆农业大学 |
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| 基金项目： | 国家高技术研究发展计划（863计划） |
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| 摘 要： | 为梨树品种改良、遗传转化及功能验证奠定基础，以杜梨(Pyrus betulaefolia)子叶外植体为试验材料，研究了基本培养基、植物生长调节剂、暗培养时间、碳源、外植体等因素对其再生能力的影响，筛选出适合杜梨不定芽分化的培养及生根培养条件，建立杜梨子叶的再生体系。结果表明，子叶诱导不定芽生长最佳培养基为NN69+6-BA5mg/L+IBA0.05mg/L+蔗糖35g/L，再生频率为100%；出芽后最佳继代培养基为MS+6-BA1.0mg/L+NAA0.1mg/L，平均再生芽数4.84；诱导不定芽生根最佳培养基为1/2MS+IBA1.0mg/L+蔗糖30g/L，生根率为80%。通过上述条件的优化，建立了杜梨子叶高效的再生体系。
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| 关 键 词： | 茶红素 茶红素 研究现状 结构 茶红素功效 |
| 收稿时间： | 2015/3/27 0:00:00 |
| 修稿时间： | 2015/6/18 0:00:00 |
Establishment of Regeneration System in Vitro for Pyrus betulaefolia Cotyledon |
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| Abstract: | In order to lay a certain foundation for the improvement of pear tree cultivars, the genetic transformation and the functional verification, Pyrus betulaefolia cotyledon explants were taken as experimental materials to establish the regeneration system. This research studied the effects of basic culture medium, plant growth regulator, dark incubation time, carbon source, explants and other factors on the regeneration of adventitious buds, screening out the suitable conditions for the cultures of the adventitious bud differentiation and the rooting of Pyrus betulaefolia. The result showed that: the best culture medium in which cotyledon induced the growth of adventitious buds was NN69 6-BA 5 mg/L IBA0.05 mg/L sucrose 35 g/L, and its regeneration frequency was 100%; after budding, the best subculture medium was MS 6-BA 1.0 mg/L NAA 0.1 mg/L, and its average bud regeneration number was 4.84; the best culture medium inducing adventitious buds to take root was 1/2 MS IBA 1.0 mg/L sucrose 30 g/L, and the rooting rate was 80%. By optimizing the above conditions, this research had established the efficient regeneration system for Pyrus betulaefolia cotyledon. |
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| Keywords: | Pyrus betulaefolia cotyledon adventitious buds regeneration system |
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