| Peatl蛋白及其3个缺失突变体的表达与热稳定性分析 |
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| 引用本文： | 李明勇,邱德文,曾洪梅,杨秀芬.Peatl蛋白及其3个缺失突变体的表达与热稳定性分析[J].农业科学与技术,2008,9(1):32-34. |
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| 作者姓名： | 李明勇 邱德文 曾洪梅 杨秀芬 |
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| 作者单位： | 李明勇（中国农业科学院植物保护研究所,北京,100081）;邱德文（中国农业科学院植物保护研究所,北京,100081）;曾洪梅（中国农业科学院植物保护研究所,北京,100081）;杨秀芬（中国农业科学院植物保护研究所,北京,100081） |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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北京市科委科技计划项目 |
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| 摘 要： | 从极细链格孢菌中提取出一种植物激活蛋白，后命名为Peatl，其分子量为35 kD。该蛋白具有良好的热稳定性。生物信息学分析显示，Peatl含有2个保守结构域，即NAC（Nascent polypeptide-associated complex）和UBA（Ubiquitin-associated）结构域。该文将Peatl在E.coli中进行克隆和表达，并构建3个缺失突变体，拟对赋予Peatl热稳定性的区域进行初步定位。
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| 关 键 词： | Peatl蛋白 突变体 热稳定性 链格孢菌 |
Expression and Thermal Stability Analysis of Peat1 and the 3 Deletion Mutants |
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| LI Ming-yong,QIU De-wen,ZENG Hong-mei,YANG Xiu-fen.Expression and Thermal Stability Analysis of Peat1 and the 3 Deletion Mutants[J].Agricultural Science ＆ Technology,2008,9(1):32-34. |
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| Authors: | LI Ming-yong QIU De-wen ZENG Hong-mei YANG Xiu-fen |
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| Institution: | (Institute of Plant Protection, CAAS ,Beijing 100081) |
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| Abstract: | Objective]The research aimed to reveal the functions of NAC and UBA domains in Peatl's thermal stability.Method]Fusion expression vectors of Peatl protein and the 3 deletion mutants were constructed.The recombinant plasmids were induced by IFI'G and the target proteins(Peat1,Peat1-△CD99,Peat1-△ND49 and Peat1-△ND108)were expressed obtained by AKTA and its thermal stability was analyzed.Result]The research found that 3 deletion mutants have good thermal stability like Pear1.Conclusion]The research demonstrated that the coexistence of NAC or UBA domains is not necessary to thermal stability of Peatl protein,and they may give the protein particular stability structure seporately. |
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| Keywords: | Petal Deletion mutant Thermal stability |
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