| 普通狗牙根种子组培技术优化及植株再生 |
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| 引用本文： | 姜茜,张琪,郑丽屏,蔡平.普通狗牙根种子组培技术优化及植株再生[J].北方园艺,2010(10):162-166. |
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| 作者姓名： | 姜茜 张琪 郑丽屏 蔡平 |
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| 作者单位： | 苏州大学,建筑与城市环境学院,江苏,苏州,215123;苏州大学,建筑与城市环境学院,江苏,苏州,215123;苏州大学,建筑与城市环境学院,江苏,苏州,215123;苏州大学,建筑与城市环境学院,江苏,苏州,215123 |
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| 基金项目： | 苏州市科技支撑(农业)资助项目 |
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| 摘 要： | 以普通狗牙根的成熟种子作外植体,通过胚性愈伤组织的发生进行植株再生。结果表明:诱导狗牙根种子产生愈伤组织的最佳培养基为:MS+2,4-D 3 mg/L+壳聚糖4 g/L;暗培养优于光培养,最高出愈率为87.0%;所诱导愈伤组织在1/2原激素浓度下继代,以MS+6-BA0.1 mg/L为分化培养基,获得了再生植株;半透明粘稠状愈伤组织的分化率最高,为63.1%。
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| 关 键 词： | 普通狗牙根 种子 组织培养 植株再生 |
Optimization of Common Bermudagrass Tissue Culture and Plantlet Regeneration |
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| JIANG Qian,ZHANG Qi,ZHENG Li-ping,CAI Ping.Optimization of Common Bermudagrass Tissue Culture and Plantlet Regeneration[J].Northern Horticulture,2010(10):162-166. |
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| Authors: | JIANG Qian ZHANG Qi ZHENG Li-ping CAI Ping |
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| Institution: | JIANG Qian,ZHANG Qi,ZHENG Li-ping,CAI Ping(School of Architecture , Urban Envieronment,Suzhou University,Suzhou,Jiangsu 215123) |
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| Abstract: | A tissue culture system for plant regeneration of common bermudagrass was developed.Using mature seeds as experimental material,explant callus was produced in the dark on MS medium containing 2,4-D 3 mg/L and chitosan 4 g/L.The highest frequencies of inducement was 87.0%.Maintaining the callus in the former conditions and medium,with the 1/2 level of 2,4-D.Then moved the to MS medium to obtain regenerated plantlets,with 6-BA 0.1 mg/L.The highest regeneration frequency was 63.1%,result in the ropy translucen... |
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| Keywords: | common bermudagrass seed tissue culture plantlet regenerated |
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