| 银条茎尖培养快繁及离体根状茎的诱导 |
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| 引用本文： | 张国裕,程智慧,李娟,周文安,王新华.银条茎尖培养快繁及离体根状茎的诱导[J].园艺学报,2004,31(1):106-108. |
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| 作者姓名： | 张国裕 程智慧 李娟 周文安 王新华 |
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| 作者单位： | ( 西北农林科技大学园艺学院, 杨凌712100 ; 陕西省武功烟叶复烤厂, 武功712200 ; 西安大鹏生物科技股份有限公司, 西安710075) |
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| 基金项目： | 中、德政府间科技合作项目 (CH -0 1/ 0 4)，国家农业科技成果转化资金项目 ( 0 2EFA2 1610 0 5 92) |
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| 摘 要： | 以银条‘二细一粗’品种为试材, 研究了蔗糖浓度、激素组合对茎尖培养和快速繁殖的影响及温度、光照、蔗糖浓度等因素对根状茎离体诱导的影响。结果表明: 茎尖培养较理想的培养基为MS + 蔗糖4 % + 6-BA 0.5 mg·L - 1 + NAA 0.1 mg·L - 1 , 成苗率可达74.0 %。茎尖增殖较适培养基为MS + 6-BA 3.0 mg·L - 1 + NAA 0.5 mg·L - 1 , 每个外植体平均可产生5.6 个芽。根状茎诱导以MS + 蔗糖10 % + 6-BA 510 mg·L - 1+ CCC 500 mg·L - 1培养基, 20 ℃全黑暗培养效果最好。
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| 关 键 词： | 银条 茎尖培养 快速繁殖 根状茎诱导 |
| 文章编号： | 0513-353X(2004)01-0106-03 |
| 收稿时间： | 2003-3-24 |
| 修稿时间： | 2003-6-6 |
Studies on Tip Meristem Culture Rapid Propagation and Induction of in Vitro Stolon of Manyflower Betony(Stachys floridana Schuttl.ex Benth.) |
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| Zhang Guoyu,Cheng Zhihui,Li Juan,Zhou Wenpan,Wang Xinhua.Studies on Tip Meristem Culture Rapid Propagation and Induction of in Vitro Stolon of Manyflower Betony(Stachys floridana Schuttl.ex Benth.)[J].Acta Horticulturae Sinica,2004,31(1):106-108. |
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| Authors: | Zhang Guoyu Cheng Zhihui Li Juan Zhou Wenpan Wang Xinhua |
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| Institution: | ( College of Horticulture , North-West Sci-Tech University of Agricultural and Forestry , Yangling 712100 , China ; Wugong Tobacco Re-baking Factory , Wugong 712200 , China , Xi-an Dapeng Biological Sci-Tech Stock Sharing Company , Ltd. Xipan , 710075 ,China) |
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| Abstract: | The factors which affect tip meristem culture , rapid propagation and the induction of in vitro stolon of manyflower Betony ( Stachys floridana Schuttl . ex Benth. ) were studied. The results showed that the optimum medium for tip meristem culture were MS + sucrose 4 % + 6-BA 0.5mg·L - 1 + NAA 0.1 mg·L - 1 with 74.0 %plant let ratio. MS supplemented with 6-BA 3.0 mg·L - 1 and NAA 0.5 mg·L - 1 was successful for propagation , each explant produced up to 5.6 seedlings. Total darkness , 20 ℃and MS medium with 6-BA 5.0 mg·L- 1 + CCC 500 mg·L - 1 were better for the induction of in vitro stolon. |
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| Keywords: | Manyflower Betony(Stachys floridana Schuttl ex Benth ) Tip meristem culture Propagation Stolon induction |
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