| 地涌金莲组织培养中的褐化抑制 |
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| 引用本文： | 侯健华,李正红,马宏,刘秀贤,万友名.地涌金莲组织培养中的褐化抑制[J].林业科学研究,2015,28(2):217-221. |
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| 作者姓名： | 侯健华 李正红 马宏 刘秀贤 万友名 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,云南昆明,650224 |
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| 基金项目： | 国家自然科学基金项目(31400559);中央级公益性科研院所基本科研业务费专项(CAFYBB2014QB045) |
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| 摘 要： | 地涌金莲组织培养中的褐化问题一直是其优良品种选育及规模化生产的技术瓶颈。为解决这一问题,以地涌金莲未成熟雄花为试材,研究了外植体消毒方式、植物生长调节剂种类及配比和不同抗褐化剂对愈伤组织褐化的影响。结果表明,未经消毒的外植体其污染率为0%,且褐化率和褐化指数均显著低于消毒后的外植体;6-BA在地涌金莲愈伤组织诱导中起主要作用,浓度为2 mg·L-1、3 mg·L-1时有利于愈伤组织的诱导,能显著降低褐化指数;添加柠檬酸+抗坏血酸(VC)混合液、表面添加VC均对地涌金莲褐化未起到明显抑制作用,姜汁和阿魏酸对抑制愈伤组织褐化有较好的效果,48.5 mg·L-1的阿魏酸能显著降低愈伤组织的褐化,使愈伤组织分化率达17.9%,较对照的2.9%提高了15%。
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| 关 键 词： | 地涌金莲 组织培养 愈伤组织 褐化 抗褐化剂 |
| 收稿时间： | 2014/10/22 0:00:00 |
Studies on Anti-Browning During Tissue Culture of Musella lasiocarpa |
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| HOU Jian-hu,LI Zheng-hong,MA Hong,LIU Xiu-xian and WAN You-ming.Studies on Anti-Browning During Tissue Culture of Musella lasiocarpa[J].Forest Research,2015,28(2):217-221. |
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| Authors: | HOU Jian-hu LI Zheng-hong MA Hong LIU Xiu-xian and WAN You-ming |
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| Institution: | Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China |
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| Abstract: | Callus necrosis during in vitro propagation is a large obstacles in breeding and large scale production of Musella lasiocarpa. To solve this problem, the effects of sterilizing methods of explants, the ratio of plant growth regulators and 4 kinds of browning inhibitors on callus browning were studied with the callus induced from its immature male flowers as the explants. The results indicated that the contamination rate of unsterilized explants was 0%, and its browning rate and browning index were all significantly lower than that of the explants after disinfection; 6-BA played a major role in callus induction of M. lasiocarpa. It conduced to callus induction and significantly reduced browning index when the concentration of 6-BA was 2 mg·L -1 or 3 mg·L -1. Adding VC on the surface and VC mixture of citric acid failed to suppress browning. However, Ginger and ferulic acid gave satisfactory result to reduced browning, 48.5 mg·L-1 ferulic acid could significantly reduce the callus browning and increased the rate of differentiation to17.9%, 15% higher than that of the control. |
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| Keywords: | Musella lasiocarpa tissue culture callus browning browning inhibitors |
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