| 一种海洋细菌显色分离培养基优化 |
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| 引用本文： | 汪 波,王伟达,李沛钿,杜 晨,颜亨梅.一种海洋细菌显色分离培养基优化[J].中国农学通报,2015,31(8):93-96. |
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| 作者姓名： | 汪 波 王伟达 李沛钿 杜 晨 颜亨梅 |
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| 作者单位： | (北京师范大学珠海分校,广东珠海 519087） |
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| 摘 要： | 针对一种海洋细菌显色分离培养基的成分进行了探究。通过改变科玛嘉海洋细菌培养基中硫代硫酸钠(0.500、0.375、0.250、0.125 g/L)，ONPG(1.5、1.0、0.5 g/L)，纤维二糖(4.0、3.0、2.0、1.0 g/L)的浓度，观察培养基中海洋细菌菌落形态，菌落面积大小和菌落颜色，统计对比出海洋细菌在不同浓度培养基上生长的状况。结果在硫代硫酸钠浓度为0.25 g/L，纤维二糖浓度为2.0 g/L，ONPG浓度为0.5~1.0 g/L时，培养基上菌落清晰，个体适中且菌落数较多，有较好的检测效果。说明该研究在保证海洋细菌显色分离培养基分离效果前提下，降低了海洋细菌显色培养基配制成本，同时，提升了其对海洋细菌的检测效果。
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| 关 键 词： | 杏鲍菇 杏鲍菇 单因素试验 原生质体 |
| 收稿时间： | 2014/7/31 0:00:00 |
| 修稿时间： | 2014/10/24 0:00:00 |
Optimization of A Marine Bacterium Chromogenic Medium |
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| Wang Bo;Wang Weida;Li Peitian;Du Chen;Yan Hengmei.Optimization of A Marine Bacterium Chromogenic Medium[J].Chinese Agricultural Science Bulletin,2015,31(8):93-96. |
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| Authors: | Wang Bo;Wang Weida;Li Peitian;Du Chen;Yan Hengmei |
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| Institution: | Wang Bo;Wang Weida;Li Peitian;Du Chen;Yan Hengmei;Beijing Normal University,Zhuhai Campus; |
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| Abstract: | A kind of marine bacteria chromogenic medium was improved to enhance effect of detection by changing the CHROMagar marine bacteria sodium thiosulfate in the culture medium (0.500; 0.375; 0.250; 0.125g/L), ONPG (1.5; 1.0; 0.5g/L), fiber two sugar (4.00; 3.0; 2.0; 1.0g/L) concentration. The results showed that there were more marine bacteria?colony numbers and more clear colony color when Sodium thiosulfate reached to 0.25g/L, Fiber two?sugar reached to 2.0g/L and ONPG reached to 0.5~1.0 g/L. It indicated that this research could reduce the cost, furthermore, it also could improve the detect effect. |
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| Keywords: | marine bacteria chromogenic medium optimization |
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