| “大通＂牦牛Lfcin基因克隆及生物信息学分析 |
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| 引用本文： | 裴杰,阎萍,姬国红,冯瑞林,梁春年,郭宪,曾玉峰,包鹏甲,褚敏.“大通＂牦牛Lfcin基因克隆及生物信息学分析[J].农业科学与技术,2009,10(3):50-54. |
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| 作者姓名： | 裴杰 阎萍 姬国红 冯瑞林 梁春年 郭宪 曾玉峰 包鹏甲 褚敏 |
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| 作者单位： | 裴杰,阎萍,冯瑞林,梁春年,郭宪,曾玉峰,包鹏甲,褚敏,PEI Jie,YAN Ping,FENG Rui-lin,LIANG Chun-nian,GUO Xian,ZENG Yu-feng,BAO Peng-jia,CHU Min(中国农业科学院兰州畜牧与兽药研究所,甘肃兰州,730050);姬国红,JI Guo-hong(甘肃农业大学草业学院,甘肃兰州,730070) |
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| 基金项目： | 中央级公益性科研院所基本科研业务费资助项目 |
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| 摘 要： | 乳铁蛋白素（Lactoferricin，Lfcin）是从乳铁蛋白（LF）上被胃蛋白酶水解下来的25个氨基酸残基的小肽，位于乳铁蛋白第二外显子，是乳铁蛋白的抗菌活性中心，其抗菌活性是LF的几百倍。Lfcin具有广谱抗菌活性，对革兰氏阳性菌和革兰氏阴性菌均有效，同时具有抗真菌、抗病毒、抑制肿瘤细胞生长、参与免疫调节等多种生物学功能和维持动物肠道菌群正常生态稳定的功能，从而起到提高动物生产性能的作用。
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| 关 键 词： | ＂大通”牦牛 Lfcin基因 克隆 生物信息学分析 |
Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak |
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| PEI Jie,YAN Ping,JI Guo-hong,FENG Rui-lin,LIANG Chun-nian,GUO Xian,ZENG Yu-feng,BAO Peng-jia,CHU Min.Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak[J].Agricultural Science ＆ Technology,2009,10(3):50-54. |
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| Authors: | PEI Jie YAN Ping JI Guo-hong FENG Rui-lin LIANG Chun-nian GUO Xian ZENG Yu-feng BAO Peng-jia CHU Min |
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| Institution: | 1. Lanzhou Institute of Animal Science and Veterinary Pharmaceutical Science, Chinese Academy of Agricultural Science, Lanzhou 730050; 2. Gansu Agricultural University, College of Grass Science, Lanzhou 730070) |
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| Abstract: | Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein. |
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| Keywords: | Yak Lfcin gene Clone Bioinformatics |
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