| 紫外分光光度法测定玉米过氧化氢酶活性新进展 |
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| 引用本文： | 王 群,刘朝巍,徐文娟.紫外分光光度法测定玉米过氧化氢酶活性新进展[J].中国农学通报,2016,32(15):159-165. |
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| 作者姓名： | 王 群 刘朝巍 徐文娟 |
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| 作者单位： | 石河子大学农学院 新疆生产建设兵团绿洲生态农业重点实验室,石河子大学农学院作物栽培教研室,石河子大学农学院 新疆生产建设兵团绿洲生态农业重点实验室 |
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| 基金项目： | 国家自然科学基金“新疆滴灌条件下高产(≥15000 kg/hm2)玉米需水规律及其调控研究”(31360276)；石河子大学高层人才项目“新疆高产(≥15000 kg/hm2)玉米需水规律及灌溉技术研究”(RCZX201217)。 |
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| 摘 要： | 为简便快速测定植物过氧化氢酶活性,探究过氧化氢酶活性随时间变化的关系,运用紫外分光光度法比较了4种反应体系中过氧化氢酶(CAT)催化H2O2分解的时间曲线。研究结果表明:CAT催化H2O2的分解反应仅在一定底物浓度范围内符合一级反应,因此计算出速率常数K,即代表CAT活性。直接以0.03%H2O2作为CAT反应液,配制简便,试剂廉价,而且得到的时间曲线效果最好,是CAT反应液的最佳选择。
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| 关 键 词： | 过氧化氢酶 紫外分光光度法 速率常数 时间曲线 反应液 |
| 收稿时间： | 2/5/2016 12:00:00 AM |
| 修稿时间： | 5/4/2016 12:00:00 AM |
Ultraviolet Spectrophotometry Measurement of Catalase Activity in Maize |
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| Abstract: | In order to simply and rapidly measure catalase (CAT) activity in plant and explore the correlation between CAT activity and time variation, ultraviolet spectrophotometry was applied to compare the time course of the decomposition of H2O2 catalyzed by CAT in four different reaction systems. The results showed that the decomposed reaction of H2O2 catalyzed by CAT accorded with the first order reaction in a certain substrate concentration. So the constant value of rate K could be represented the activity of CAT. Compared with other reaction solutions, 0.03% H2O2 had such advantages as simple preparation, low cost and could get the best time course in the measurement, so it was the optimum reaction solution of CAT activity measurement. |
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| Keywords: | catalase ultraviolet spectrophotometry the rate constant time course reaction solution |
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