| 纯化黑加仑种子中超氧化物歧化酶(SOD)的研究 |
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| 引用本文： | 侯丽华,明霞.纯化黑加仑种子中超氧化物歧化酶(SOD)的研究[J].沈阳农业大学学报,2004,35(2):133-134. |
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| 作者姓名： | 侯丽华 明霞 |
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| 作者单位： | 沈阳农业大学,辽宁,沈阳,110161 |
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| 摘 要： | 采用有机溶剂分级沉淀法、纤维素离子交换层析法及葡聚糖凝胶过滤法从黑加仑种子中提取SOD。实验证明 :粗酶液经上述方法纯化后SOD酶活性达692.95U,蛋白含量为0.72mg,比活达964540U·g-1,纯化倍数达253.16 ,回收率为31.41%。黑加仑种子中的SOD是Cu.Zn-SOD
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| 关 键 词： | 黑加仑种子 超氧化物歧化酶 纯化技术 |
| 文章编号： | 1000-1700(2004)02-0133-02 |
| 修稿时间： | 2003年12月17 |
Separation and Purification of SOD from the Black Currant Seeds |
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| HOU Li-hua,MING Xia.Separation and Purification of SOD from the Black Currant Seeds[J].Journal of Shenyang Agricultural University,2004,35(2):133-134. |
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| Authors: | HOU Li-hua MING Xia |
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| Abstract: | Separation and purification of superoxide dismutase(SOD)from the seeds of black currant were made with the methods of organic agent fractionation,Ion-exchange chromatography and Gel-filtration chromatography.The results showed that the total activity was692.95U,total protein was0.72mg,specific activity was964.54U/mg.pr,purification fold was253.16,yield was31.41%.SOD from black currant seeds is Cu.Zn-SOD. |
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| Keywords: | black currant seeds SOD purification techonology |
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