| 亚洲1型口蹄疫病毒L蛋白缺失株的构建及其生物学定性 |
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| 引用本文： | 薛美,王海伟,于力.亚洲1型口蹄疫病毒L蛋白缺失株的构建及其生物学定性[J].中国预防兽医学报,2010,32(4). |
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| 作者姓名： | 薛美 王海伟 于力 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 |
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| 基金项目： | 黑龙江省科技攻关重大项目专题 |
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| 摘 要： | 为确定口蹄疫病毒(FMDV)L蛋白缺失对其生物学特性的影响,本研究在已建立的Asia1型FMDV反向遗传操作平台基础上,利用融合PCR方法缺失L蛋白基因编码区,构建了缺失L蛋白的重组Asia1型FMDV(rFMDV-ΔL)。拯救病毒的一步生长曲线结果表明,rFMDV-ΔL与亲本病毒相比在BHK-21细胞中的复制水平下降10倍,乳鼠毒力试验表明rFMDV-ΔL的毒力比亲本毒株下降6倍。另外,rFMDV-ΔL在BHK-21细胞上产生病变的速度明显变慢,形成蚀斑的时间延长,致乳鼠死亡所需时间也比亲本毒株明显延长。本研究是关于亚洲1型FMDVL蛋白结构与功能研究的首次报道,为亚洲1型FMDVL蛋白结构与功能的研究奠定了基础。
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| 关 键 词： | Asial型口蹄疫病毒 L蛋白缺失 重组病毒拯救 生物学定性 |
Construction and biological characterization of leader-deleted Asia1 foot-and-mouth disease virus |
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| XUE mei,WANG Hai-wei,YU Li.Construction and biological characterization of leader-deleted Asia1 foot-and-mouth disease virus[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(4). |
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| Authors: | XUE mei WANG Hai-wei YU Li |
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| Institution: | XUE mei,WANG Hai-wei,YU Li(Division of Livestock Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | By using overlapping PCR, a recombinant food and mouth disease virus (FMDV) with L gene delated (rFMDV-ΔL) was generated based on FMDV Asial reverse genetic system. Comparing with parental virus, the replication of rFMDV-ΔL was 10-fold lower in BHK-21 cells, and pathogenicity to mice was reduced by 6-fold. Moreover, there was a delay in spread of the rFMDV-ΔL in mice, consistent with the observation that virus replication in virus-infected BHK-21 cells were delayed relative to wild-type virus. This is the first report on studies of the structure and function of the leader (L) proteinase of serotype Asial FMDV. |
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| Keywords: | foot-and-mouth disease virus serotype Asial deletion of leader (L) proteinase rescue recombinant virus biological characterization |
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