Characterization of Prunus domestica L. in vitro regeneration via hypocotyls |
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| Authors: | Lining Tian Susan Sibbald Jayasankar Subramanian Antonet Svircev |
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| Institution: | 1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario, Canada N5V 4T3;2. Department of Plant Agriculture-Vineland Station, University of Guelph, 4890 Victoria Avenue, North, Vineland Station, Ontario, Canada L0R 2E0;3. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 4902 Victoria Avenue, North, Vineland Station, Ontario, Canada L0R 2E0 |
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| Abstract: | Several important aspects of regeneration of European plum from hypocotyl explants were studied. Multiple shoots were induced and full plants were recovered for a large number of plum varieties. This indicates that European plum species is, in general, very responsive to in vitro regeneration from hypocotyls. Shoot organogenesis could be induced from both mature and immature seed explants but regeneration efficiency was higher when immature seeds were used. Rooting efficiency for varieties with low rooting tendency could be greatly increased by addition of naphthaleneacetic acid in the medium. Primary shoots, when sub-cultured on fresh induction medium, produced multiple shoots at a high frequency and such multiplication could continue for many cycles. The secondary new shoots could be induced for various plum varieties. Plant recovery from the secondary shoots was as efficient as that from the primary shoots. This new system may be an alternative for plum transformation with a potential for increasing transformation efficiency. The system can be used for propagation of transgenic lines and other genetic clones of various varieties. |
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| Keywords: | IBA indolebutyric acid NAA naphthaleneacetic acid TDZ thidiazuron |
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