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Discrimination of the grapevine cultivars ‘Picolit’ and ‘Kéknyel?’ with molecular markers
Authors:G Jahnke  J Korbuly  J Májer  J Györffyné Molnár
Institution:1. FVM Research Institute for Viticulture and Enology, Badacsony, Hungary;2. National Institute for Agricultural Quality Control, Budapest, Hungary
Abstract:The name of the grapevine cultivar ‘Kéknyel?’ has become inseparable from the name of the Badacsony vine region, whose fame is well known beyond our frontier as well. In the Vitis International Variety Catalogue (http://www.genres.de/idb/vitis/) ‘Kéknyel?’ is reported, as the synonym of the Italian grapevine cultivar ‘Picolit’. Vertical poliacrylamide-gel electrophoresis was used for the investigation isoenzymes of catechol-oxidase (CO) and acid phosphatase (AcP). Microsatellite analyses were carried out at 6 loci (VVS2, VVS16, VrZag79, VVMD7, VMC4A1, VMC4G6). The results of the isoenzymatic and microsatellite analyses confirmed, that this two cultivars are different.
Keywords:Isoenzyme  SSR  Microsatellite  Cultivar identification  PAGE  PCR  Grape    knyel?  Picolit
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