Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers |
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| Authors: | B Shiran N Amirbakhtiar S Kiani Sh Mohammadi BE Sayed-Tabatabaei H Moradi |
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| Institution: | 1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, P.O. Box 115, Shahrekord, Iran;2. Department of Agro Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 84154-83111, Iran;3. Section of Horticulture, Agriculture and Natural Resources Research Center of Shahrekord, P.O. Box 88155-415, Iran |
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| Abstract: | RAPDs and SSRs were used to study the genetic diversity of Iranian almond cultivars and their relationship to important foreign cultivars and three related species. Eight unidentified almond Shahrodi cultivars and three wild almonds (Prunus communis, Prunus orientalis and Prunus scoparia) were also included. Of the primers tested, 42 (out of 80) RAPD and 18 (out of 26) SSR primers were selected for their reproducibility and high polymorphism. A total of 664 polymorphic RAPD bands were detected out of 729 bands. The number of presumed alleles revealed by the SSR analysis ranged from 3 to 10 alleles per locus with a mean value of 6.64 alleles per locus. Both techniques discriminated the genotypes very effectively, but only RAPDs were able to discriminate the cultivars Monagha and Sefied. Results demonstrated an extensive genetic variability within the tested cultivars as well as the value of SSR markers developed in peach for characterization of almond and related species of Prunus. Dice similarity coefficient was calculated for all pair wise comparisons and was used to construct a UPGMA dendrogram. For both markers a high similarity in dendrogram topologies was obtained although some differences were observed. All dendrograms, including that obtained by the combined use of both the marker data, depicted the phenetic relationships among the cultivars and species, depending upon their geographic region and/or pedigree information. Almond cultivars clustered with accession of P. communis showing their close relationship. P. orientalis and P. scoparia were clustered out of the rest of P. dulcis. |
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| Keywords: | RAPDs SSRs Molecular markers Almond Prunus dulcis Genetic relationships |
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