Genetic Variation of Glutelin Acidic Subunit Polypeptides in Bangladesh Rice Genetic Resources |
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| Authors: | Md Sarwar?Jahan Yuji?Uemura Toshihiro?Kumamaru Abdul?Hamid Email author" target="_blank">Hikaru?SatohEmail author |
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| Institution: | (1) Plant Genetics Laboratory, Institute of Genetic Resources, Kyushu University, Hakozaki 6-10-1, 812-8581 Higashi-ku, Fukuoka, Japan;(2) Department of Agronomy, Bangabandhu Seikh Mujibur Rahman Agricultural University, 1703 Salna, Gazipur, Bangladesh;(3) Present address: Agrotechnology Discipline, Khulna University, Khulna, 9208, Bangladesh |
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| Abstract: | Bangladesh rice genetic resources collected from six distinct regions of the country were examined to obtain the diversity
in glutelin acidic subunit polypeptides. Seed glutelins from 576 Bangladesh rice cultivars representing seven ecotypes were
analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF) electrophoresis
and two-dimensional electrophoresis (2-DE: SDS-PAGE/IEF) analyses. Glutelin acidic subunit was separated into four bands,
α-1, α-2, α-3 and α-4, and the variation in each of bands was detected by SDS-PAGE analysis. A higher molecular size component of α-1(α-1′) was identified in the cultivars tested. In case of α-2, α-2H with high molecular mass, α-2L with low molecular mass and α-2H/L were detected. α-3 band variation showed α-3H, α-3L, and α-3H/L, while for α-4, α-4H, α-4L bands were identified. In IEF analysis, a total of 16 bands with independent pI ranging from pI 6.30 to 7.52 were identified for the glutelin acidic subunit among the cultivars. The maximum and minimum numbers of IEF
bands found were 13 and 9, respectively. The α-2L less cultivars were also lacking in pI 6.80 polypeptide in IEF. Result of 2-DE showed that pI 6.80 polypeptide was the main component of α-2L band. Transplanted Aman ecotype was the most diverse with respect to glutelin variation. Geographical distribution of
glutelin variation in the Transplanted Aman ecotype differed according to the regions. The above results indicate that Bangladesh
rice cultivars possess great genetic diversity in glutelin acidic subunit polypeptides. This study of indigenous rice cultivars
from Bangladesh provides useful information regarding their breeding potential. |
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| Keywords: | Ecotype Electrophoresis Oryza sativa L Seed Storage protein |
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