| 轮叶党参叶片的组织培养研究 |
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| 引用本文： | 彭金环,张美珍,于元杰.轮叶党参叶片的组织培养研究[J].特产研究,2010,32(1):29-31,34. |
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| 作者姓名： | 彭金环 张美珍 于元杰 |
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| 作者单位： | 山东农业大学农学院,山东,泰安,271018 |
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| 摘 要： | 以轮叶党参叶片为外植体,研究了培养基及不同激素组合对愈伤组织诱导及植株再生的影响。结果表明,叶片作为外植体可以比较容易地诱导出愈伤组织,最佳诱导培养基为MS+2,4-D 1.0mg/L+6-BA 1.0mg/L+KT 0.5mg/L,最高出愈率为94.2%;诱导不定芽的最佳培养基为MS+6-BA 2.0mg/L+NAA 0.5mg/L,最高诱导率可达69.2%;根诱导的最佳培养基为MS+6-BA1.0mg/L+NAA 0.5mg/L,生根率达85.9%,建立了较为完善的轮叶党参再生体系。
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| 关 键 词： | 轮叶党参 愈伤组织 诱导 |
Study on Tissue Culture Using Leaves of Codonopsis lanceolata Benth |
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| PENG Jin-huan,ZHANG Mei-ling,YU Yuan-jie.Study on Tissue Culture Using Leaves of Codonopsis lanceolata Benth[J].Special Wild Economic Animal and Plant Research,2010,32(1):29-31,34. |
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| Authors: | PENG Jin-huan ZHANG Mei-ling YU Yuan-jie |
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| Institution: | PENG Jin-huan,ZHANG Mei-ling,YU Yuan-jie(College of Agronomy,Sh,ong Agricultural University,Taian 271018,China) |
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| Abstract: | The leaves were used as explants to study the influence of different hormone combinations on callus induction and plant re-generation of Codonopsis lanceolata Benth.The results showed that the optimal medium for intensive and rapid growth of callus was MS medium supplemented with 1.0mg/L 2,4-D and 2.0mg/L KT.The highest callus-forming rate reached 94.2%;the optimal medium for regenerated seedlings was MS medium supplemented with 2.0mg/L 6-BA and 0.5mg/L NAA.The highest induction rate reached 69.2% while MS ... |
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| Keywords: | Codonopsis lanceolata Benth callus induction |
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