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云南保种乳用圭山羊遗传多样性蛋白电泳研究
引用本文:苗永旺,宿兵.云南保种乳用圭山羊遗传多样性蛋白电泳研究[J].云南农业大学学报,1997,12(3):199-205.
作者姓名:苗永旺  宿兵
作者单位:[1]云南农业大学动物科学技术学院 [2]中国科学院昆明动物研究所
基金项目:云南省应用基础研究重大项目
摘    要: 采用水平式淀粉胶蛋白电泳技术,对路南保种乳用圭山羊33个个体的46个遗传座位的血液蛋白及同工酶的多态性进行了研究.结果发现AKP,LAP,CES-I,ESD,ME和Pa6个座位具多态性,多态座位的基因AKP0,LAPA,CES-I1,MEB,ESD1和PaA的基因频率较高;多态座位百分比P=0.1304,平均杂合度H=0.0501.在Tf座位,出现两种表型AA,AB,但多态性贫乏;LDH谱带中发现一种不同的类型.结果表明,该品种山羊与已检测的其它山羊比较,蛋白多态水平较高,遗传背景较丰富.

关 键 词:路南保种乳用圭山羊  遗传多样性  蛋白及同工酶电泳
收稿时间:1996-12-24

Study on the Blood Protein and Isozyme Polymorphism of the Conserved Gui Dairy Goat in Yunnan
Miao Yongwang,Ye Shaohui,Wang Xia,Peng Helu,Su Bing.Study on the Blood Protein and Isozyme Polymorphism of the Conserved Gui Dairy Goat in Yunnan[J].Journal of Yunnan Agricultural University,1997,12(3):199-205.
Authors:Miao Yongwang  Ye Shaohui  Wang Xia  Peng Helu  Su Bing
Institution:Miao Yongwang 1 Ye Shaohui 1 Wang Xia 1 Peng Helu 1 Su Bing 2
Abstract:Using the horizontal starch gel electrophoresis, protein and isozyme polymorphism of thirty-three blood samples from the three populations of conserved Gui dairy goat were analyzed Out of 46 loci assayed, polymorphism was found at the following six loci: AKP, LAP, CES-I, ME, ESD and Pa The other loci were monomorphic Gene frequencies of polymorphic loci were calculated and found high in AKP 0, LAP A, CES-I 1, ME B , ESD 1 and Pa A The genetic variation at Tf locus was poor; we could find only two heterozygous(AB) individuals in thirty-three samples The Particular phenotype of the Lactate Dehydrogenas (LDH)was detected in the samples The genetic variation in conserved Gui dairy goat was high compared with other goat populations so far examined The proportion of polymorphic loci(P)and the average heterozygosity per individual(H) were estimated as 0 1304, 0 0501, respectively The results showed that the Conserved Gui dairy goat is wealthy in genetic diversity
Keywords:Conserved Gui Dairy Goat  Protein electrophoresis  Genetic diversity
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