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| RAPD和SSR标记检测海南普通野生稻籼粳分化的比较研究 | |
| 引用本文： | 崔莹莹,郭安平,王晓玲,孔华,郭运玲,贺立卡.RAPD和SSR标记检测海南普通野生稻籼粳分化的比较研究[J].热带作物学报,2009,30(6):773-780. |
| 作者姓名： | 崔莹莹 郭安平 王晓玲 孔华 郭运玲 贺立卡 |
| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;海南大学农学院,海口,571101 2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 3. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;江西省农业科学院水稻研究所,南昌,330200 |
| 基金项目： | 农业部农业结构调整重大技术研究专项，中央级公益性科研院所基本科研业务费热区转基因植物生物安全及生物多样性研究和中央级公益性科研院所基本科研业务费 |
| 摘 要： | 应用RAPD和SSR分子标记技术分别对来自海南省的51份普通野生稻样品籼梗分化情况进行研究.结果表明,40条RAPD引物中筛选出具有多态性的引物15条,共扩增出具有多态性标记位点96个,占总位点的78.05%,所用引物多态性信息量(PIC)平均值为0.720.28对SSR引物中筛选出条带清晰且多态性高的22对引物,共扩增出188个标记位点,其中172个具有多态性,占总位点的92.10%,SSR引物的PIC平均值为0.794.显然,SSR检测出多态性条带的能力优于RAPD.RAPD和SSR标记的分子聚类获得了趋势相近但不完全相同的聚类树状图.聚类结果表明.2种标记均揭示了海南普通野生稻具有一定程度的籼粳分化.其中偏粳多于偏籼.本研究为进一步明确海南普通野生稻在中国栽培稻起源演化上的地位.并对海南普通野生稻资源的发掘利用提供理论依据. |
| 关 键 词： | 普通野生稻 籼粳分化 |
RAPD and SSR Detection of Indica-Japonica Differentiation in Common Wild Rice(Oryza rufipogon)in Hainan |
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| Cui Yingying,Guo Anping,Wang Xiaoling,Kong Hu,Guo Yunling and He Lika.RAPD and SSR Detection of Indica-Japonica Differentiation in Common Wild Rice(Oryza rufipogon)in Hainan[J].Chinese Journal of Tropical Crops,2009,30(6):773-780. | |
| Authors: | Cui Yingying Guo Anping Wang Xiaoling Kong Hu Guo Yunling and He Lika |
| Institution: | Cui Yingying1,2,Guo Anping1,Wang Xiaoling1,3,Kong Hua1,Guo Yunling1,He Lika1 1 Institute of Tropical Bioscience , Biotechnology,Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou,571101,2 College of Agronomy,Hainan University,3 Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang,330200 |
| Abstract: | Fifty one individual samples of common wild rice(Oryza rufipogon) collected from Hainan were detected by using the RAPD and SSR techniques to analyze their potential differentiation into indica and japonica types of cultivated rice. From 40 RAPD arbitrary primers were screened 15 effective primers, and a total of 96 polymorphic fragments amplified (78.05% polymorphism), with the polymorphic information content(PIC) being 0.720 by average. From 28 SSR primers were screened 22 effective primers, and a total o... |
| Keywords: | RAPD SSR |
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