| 薄层色谱和高效液相色谱联合检测玉米赤霉烯酮的方法研究 |
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| 引用本文： | 吴文达,王宝杰,蔡兰芬,何成华,李治忠,王希春,刘海明,施志玉,张海彬.薄层色谱和高效液相色谱联合检测玉米赤霉烯酮的方法研究[J].畜牧与兽医,2010,42(7). |
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| 作者姓名： | 吴文达 王宝杰 蔡兰芬 何成华 李治忠 王希春 刘海明 施志玉 张海彬 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095
2. 山东畜牧兽医职业学院,山东,潍坊,261061 |
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| 基金项目： | 国家高技术研究发展计划 |
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| 摘 要： | 建立了薄层色谱法(TLC)定性检测和高效液相色谱法(HPLC)定量检测相结合的检测玉米粉中玉米赤霉烯酮(ZON)的方法。以石油醚∶乙醚∶冰醋酸(70∶28∶2)为展开剂,TCL定性检测ZON,ZON的比移值(Rf值)约为0.26。HPLC对ZON进行定量分析,ZON在0.25~5μg/mL范围内线性关系良好,回收率高,检测限为3μg/kg。试验表明:TLC快速、简便,可作为大批量、快速检测ZON的有效方法;HPLC精确、灵敏度高可作为定量检测ZON的有效手段。
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| 关 键 词： | 玉米赤霉烯酮 检测 TLC HPLC |
Detection of zearalenone by thin layer chromatography combined with high performance liquid chromatography |
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| WU Wen-da,WANG Bao-jie,CAI Lan-fen,HE Cheng-hua,LI Zhi-zhong,WANG Xi-chun,LIU Hai-ming,SHI Zhi-yu,ZHANG Hai-bin.Detection of zearalenone by thin layer chromatography combined with high performance liquid chromatography[J].Animal Husbandry & Veterinary Medicine,2010,42(7). |
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| Authors: | WU Wen-da WANG Bao-jie CAI Lan-fen HE Cheng-hua LI Zhi-zhong WANG Xi-chun LIU Hai-ming SHI Zhi-yu ZHANG Hai-bin |
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| Abstract: | Thin layer chromatography(TLC) combined with high performance liquid chromatography(HPLC) were used for the detection of zearalenone(ZON) in corn flour.Rf value of ZON for TLC with ligarine-ethylether-glacial acetic acid(70∶28∶2,V/V) as expanded material was determined as 0.26.Quantitation of ZON was carried out by HPLC.The linear correlation was well between 0.25～5 μg/mL.The coefficient of recovery was high and detection limit was 3 μg/kg.It suggested that TLC is fast and convenient,and suitable for quick detection in a large batch,while HPLC is precise and sensitive,and suitable for quantitation of ZON. |
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| Keywords: | TLC HPLC |
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