| 用二次正交旋转组合设计优化马占相思增殖培养基 |
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| 引用本文： | 徐位力,苏开君,王伟平,王光,李杰.用二次正交旋转组合设计优化马占相思增殖培养基[J].广东林业科技,2003,19(4):13-16. |
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| 作者姓名： | 徐位力 苏开君 王伟平 王光 李杰 |
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| 作者单位： | 广州市林业科学研究所,广州,510515 |
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| 基金项目： | 广州市科委资助项目，编号 (ZB0 2 ) 98 Z 0 0 2 0 1 |
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| 摘 要： | 在马占相思组织培养初步取得成功的基础上，采用二次正交旋转组合设计对马占相思增殖培养基进行优化，建立增殖率(Y)对Ca^2 浓度(X1)、6-BA浓度(X2)及NAA浓度(X3)3个试验因子的正交回归模型：Y=2．280-0．168X1-0．259X2 0．185X1^2-0．210X2^2 0．167X3^2 0．326X1X2。从模型推知，当Ca^2 浓度为0．58倍常规MS培养基浓度(255g／L)，6BA为0．76mg／L，NAA为0．16mg／L时，增殖率达最大值为4．32，实验结果与预测值相符。
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| 关 键 词： | 马占相思 增殖培养基 优化 二次正交旋转组合设计 生物量 |
Optimize the Multiplication Medium of Acacia mangium through the Quadratic Orthogonal Rotation Combination Design |
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| XU Weili,Su Kaijun,Wang Weiping,Wang Guang,Li Jie.Optimize the Multiplication Medium of Acacia mangium through the Quadratic Orthogonal Rotation Combination Design[J].Forestry Science and Technology of Guangdong Province,2003,19(4):13-16. |
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| Authors: | XU Weili Su Kaijun Wang Weiping Wang Guang Li Jie |
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| Abstract: | Based on the primary studies on tissue culture of Acacia mangium,the multiplication medium of Acacia mangium was optimized with quadratic orthogonal rotation combination design. The quadratic orthogonal regression model of multiplication rate(Y)to three factors concentration of Ca 2+ (X 1),6-BA(X 2)and NAA(X 3)was established as y2.280-0.168X 1-0.259X 2+0.185X2 1-0.210X2 2+0.167X2 3+0 326X 1X 2. It was concluded from the model that when the concentration of Ca 2+ (X 1),6-BA(X 2)and NAA(X 3)was 255 mg/L,0.76 mg/L and 0.16 mg/L respectively, the multiplication rate (Y) reach its maximum (4.32) which was consistent with the experiment result. |
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| Keywords: | Acacia mangium culture medium quadratic optimization orthogonal rotation combination design |
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