| 玉米草（Zea Mexicana）与海藻寡糖联合修复石油烃污染土壤的研究 |
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| 引用本文： | 唐景春,王斐,褚洪蕊,白晓瑞,王敏,周启星.玉米草（Zea Mexicana）与海藻寡糖联合修复石油烃污染土壤的研究[J].农业环境保护,2010(11):2107-2113. |
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| 作者姓名： | 唐景春 王斐 褚洪蕊 白晓瑞 王敏 周启星 |
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| 作者单位： | 南开大学环境科学与工程学院,环境污染过程与基准教育部重点实验室,天津300071 |
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| 基金项目： | 国家高技术研究发展计划项目（863计划 2007AA061201）; 天津市应用基础及前言技术研究计划（09JCYBJC08800）; 水体污染控制与治理重大专项（2008ZX07314-001-05） |
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| 摘 要： | 采用玉米草及海藻寡糖联合修复技术研究了石油污染土壤的修复效果,对修复过程中酶活性变化进行了测定,并采用变性梯度凝胶电泳（DGGE）技术测定了土壤中微生物群落的变化。结果表明,种植玉米草可以有效提高土壤中石油烃的降解,与对照相比石油烃降解率增加了11%;加入不同浓度海藻寡糖进一步增加了石油烃的降解效果,降解率最高达到28.6%。种植植物及加入海藻寡糖可以有效提高多酚氧化酶、脱氢酶及尿酶的活性。PCR-DGGE结果表明植物种植及海藻寡糖的加入增加了土壤中微生物数量,其微生物群落结构与未种植植物及修复前土壤相比发生了较大的变化。
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| 关 键 词： | 石油烃 修复 玉米草 海藻寡糖 变性梯度凝胶电泳（DGGE） |
Bioremediation of Petroleum Contaminated Soils by Combination of Zea Mexicana and Alginate Oligosaccharides |
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| TANG Jing-chun,WANG Fei,CHU Hong-rui,BAI Xiao-rui,WANG Min,ZHOU Qi-xing.Bioremediation of Petroleum Contaminated Soils by Combination of Zea Mexicana and Alginate Oligosaccharides[J].Agro-Environmental Protection,2010(11):2107-2113. |
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| Authors: | TANG Jing-chun WANG Fei CHU Hong-rui BAI Xiao-rui WANG Min ZHOU Qi-xing |
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| Institution: | (College of Environmental Science and Engineering,Nankai University /Key Laboratory of Pollution Processes and Environmental Criteria,Ministry of Education,Tianjin 300071,China) |
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| Abstract: | Phytoremediation is an important approach for the bioremediation of petroleum hydrocarbon contaminated soil.Alginate oligosaccharide,the product from alginate decomposition,is effective in enhancing plant growth.In this paper,combined effects of Zea Mexicana and alginate oligosaccharides on bioremediation of petroleum contaminated soil was studied.The enzyme activity of polyphenol oxidase,dehydrogenase and urease was tested and the microbiology community change in the soil was characterized by the PCR-DGGE method.The degradation rate of petroleum hydrocarbons could be improved by planting Zea Mexicana,with 11% higher degradation rate as compared to the control.Addition of different contents of oligosaccharides further increased the degradation of petroleum hydrocarbons,with maximum degradation rate of 28.6% after 3 months.The best remediation was achieved by addition of alginate oligosaccharide solution at dilution rate of 20 times.Planting and addition of seaweed oligosaccharides could effectively improve the activity of polyphenol oxidase,dehydrogenase and urease.Positive correlation was found between petroleum degradation and activity of dehydrogenase and urease,which indicated the important role of enzyme activity on the bioremediation process.PCR-DGGE results suggested that planting and alginate oligosaccharides addition to the soil had enhanced the growth of soil microorganisms,the microbial community structure had changed as compared to the soil without planting and that before remediation.The microbial community of soils with alginate oligosaccharide addition was classified into the same group in cluster analysis on the DGGE results. |
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| Keywords: | petroleum hydrocarbons remediation Zea Mexicana alginate oligosaccharides Denaturing Gradient Gel Elecrophoresis（DGGE） |
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