| 5种重金属暴露对斑马鱼呼吸运动的影响 |
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| 引用本文： | 汪红军,李嗣新,周连凤,郑金秀,梁友光.5种重金属暴露对斑马鱼呼吸运动的影响[J].农业环境保护,2010(9):1675-1680. |
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| 作者姓名： | 汪红军 李嗣新 周连凤 郑金秀 梁友光 |
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| 作者单位： | 水利部中国科学院水工程生态研究所,武汉430079 |
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| 基金项目： | 水利部“948”项目（200709）; 国家水专项--流域水环境预警技术研究与三峡库区示范项目（2009ZX07528-003-03）致谢：彭建华研究员对论文进行了修改,在此致谢. |
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| 摘 要： | 为了探讨外源性重金属暴露对鱼类呼吸反应的影响,利用鱼类的呼吸参数的变化来预警水体污染。尝试以斑马鱼为实验生物,进行了5种重金属离子对斑马鱼的急性毒性实验;应用生物早期预警系统（BIO-SENSOR 7008）,研究了斑马鱼在不同重金属浓度暴露1 h后的呼吸反应变化情况及预警结果。结果表明,Hg^2＋、Cu^2＋、Cd^2＋、Zn^2＋、Pb^2＋等重金属对斑马鱼的96 hLC50分别为0.14、0.174、6.497、44.48、116.432 mg.L-1;安全浓度分别为0.014、0.017、0.65、4.5、11.6 mg.L^-1;应用呼吸参数的预警浓度分别为0.08、0.08、4.8、7.5、105 mg.L^-1。其中系列浓度Hg2＋暴露下斑马鱼呼吸频率和呼吸强度都显著升高（F=2.75,P〈0.05;F=28.95,P〈0.01）;Cu^2＋、Cd^2＋等暴露对斑马鱼呼吸频率和呼吸强度先升高再降低（P〈0.01）;Zn^2＋暴露则对斑马鱼呼吸运动具有显著抑制作用（P〈0.01）;而Pb2＋对斑马鱼呼吸强度影响较小（P〉0.05）。斑马鱼呼吸参数作为生物监测指标来预警水体Hg^2＋、Cu^2＋、Zn^2＋等重金属污染较为敏感。
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| 关 键 词： | 斑马鱼 呼吸反应 重金属污染 生物早期预警 |
The Effect of Exposure to Five Kinds of Heavy Metals on Respiratory Movement of Zebra Fish（Brachydanio rerio） |
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| WANG Hong-jun,LI Si-xin,ZHOU Lian-feng,ZHENG Jin-xiu,LIANG You-guang.The Effect of Exposure to Five Kinds of Heavy Metals on Respiratory Movement of Zebra Fish（Brachydanio rerio）[J].Agro-Environmental Protection,2010(9):1675-1680. |
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| Authors: | WANG Hong-jun LI Si-xin ZHOU Lian-feng ZHENG Jin-xiu LIANG You-guang |
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| Institution: | (Institute of Hydroecology,MWR and CAS,Wuhan 430079,China) |
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| Abstract: | Changes in the respiratory system of fish can rapidly reflect changes in the body′s own function,which is sensitive to the external environment,reflecting the important indicators of body function state.In order to discuss the influence of exogenous heavy metal exposure on fish ventilatory response,fish ventilatory parameters were used to warn water pollution as sensitive biological marks.Trying zebra fish（Brachydanio rerio）as a experimental organism,an acute toxic experiment was carried out to study the influence of five kinds of heavy metal ions on zebra fish.By biological early warning system（BIO-SENSOR 7008）,we studied the variation of ventilatory response and warning re-sults of zebra fish exposed to different heavy metal concentrations 1 hour later.The results indicated that 96 hLC50 of Hg2＋、Cu2＋、Cd2＋、Zn2＋、 Pb2＋ on zebra fish were 0.14 、0.174 、6.497、44.48 mg.L-1 and 116.432 mg.L-1,respectively.Safe concentrations were 0.014、0.017、0.65、4.5 mg.L-1 and 11.6 mg.L-1,respectively.Warning concentrations of applying ventilator parameters were 0.08、0.08、4.8、7.5 mg.L-1 and 105 mg.L-1,respectively.Ventilatory frequency and amplitute of zebra fish exposed to a series of concentrations significantly increased（F=2.75,P〈0.05;F=28.95,P〈0.01）.Ventilatory frequency and amplitute of zebra fish first increased and then decreased exposing to Cu2＋、Cd2＋.The exposure of Zn2＋ inhibited significantly the ventilatory of zebra fish（P〈0.01）.However,Pb2＋ had little effect on the zebra fish ventilatory（P 〉0.05）.The order of zebra fish sensitivity to the five kinds of heavy metals was Cu2＋〉Hg2＋〉Zn2＋〉Cd2＋〉Pb2＋.As biological marks,zebra fish ven-tilatory parameters is sensitive to warn the pollution of Cu2＋、Hg2＋、Zn2＋ and so on in water. |
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| Keywords: | zebra fish ventilatory response heavy metal pollution biological early warning |
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