Natural infection of banana by a satellite-containing strain of cucumber mosaic virus: nucleotide sequence of the coat protein gene and the satellite RNA |
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| Authors: | R Gafny A Wexler M Mawassi Y Israeli M Bar-Joseph |
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| Institution: | 1. The S. Tolkowsky Laboratory, Dept. of Virology, ARO, The Volcani Center, 50250, Bet Dagan, Israel
2. Jordan Valley Banana Research Station, 15132, Zemach, Israel
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| Abstract: | Banana plants expressing mosaic symptoms, from the Jordan Valley in Israel, were shown to be infected by a satellite-RNA-containing strain of cucumber mosaic virus (CMV). Double-stranded RNA isolated from field-infected banana, without passage through another host, was used as a template for synthesis of cDNA. The cDNAs corresponding to the coat protein (CP) gene and to the satellite RNA were cloned after polymerase chain reaction amplification. The nucleotide sequences of the CP and the satellite cDNAs were determined. The CP gene and its 3′ flanking sequence had 98% similarity to the CMV Fny nucleotide sequence and the two strains differed in only one amino acid of the CP. The associated satellite had a sequence similarity ranging from 95% to 85.6% with other CMV satellites. Analysis of banana suckers differing in symptoms’ severity indicated a correlation between the presence of satellite and attenuation of symptoms. |
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