| 沈阳市新民地区番茄黄化曲叶病毒分子检测 |
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| 引用本文： | 苗则彦,白元俊,李颖,赵杨.沈阳市新民地区番茄黄化曲叶病毒分子检测[J].黑龙江农业科学,2014(12):77-78. |
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| 作者姓名： | 苗则彦 白元俊 李颖 赵杨 |
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| 作者单位： | 辽宁省农业科学院辽宁省农作物有害生物控制重点实验室,辽宁沈阳,110161 |
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| 基金项目： | 辽宁省农业综合开发省级重点科技推广资助项目(SNF-2014-A-01) |
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| 摘 要： | 为了鉴定并防治番茄黄化曲叶病毒病,采用分子生物学方法对沈阳新民地区3个番茄植株样品进行检测,对PCR产物中的特异性片段进行回收、克隆、测序。结果表明:3个番茄植株样品均感染番茄黄化曲叶病毒病,说明沈阳市新民地区已有番茄黄化曲叶病毒病发生且PCR技术可以快速、准确、高效地完成番茄黄化曲叶病毒病的检测。
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| 关 键 词： | 番茄 番茄黄化曲叶病毒病 分子检测 |
Molecular Identification of Tomato Yellow Leaf Curl Virus from Xinmin Area of Liaoning Province |
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| MIAO Ze-yan
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BAI Yuan-jun
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LI Ying
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ZHAO Yang.Molecular Identification of Tomato Yellow Leaf Curl Virus from Xinmin Area of Liaoning Province[J].Heilongjiang Agricultural Science,2014(12):77-78. |
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| Authors: | MIAO Ze-yan BAI Yuan-jun LI Ying ZHAO Yang |
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| Institution: | (Liaoning Academy of Agricultural Sciences, Liaoning Key Laboratory of Crop Pest Manage ment, Shenyang, Liaoning 110161) |
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| Abstract: | In order to authenticate and control tomato yellow leaf curl virus,3tomato samples were detected through molecular identification of tomato yellow leaf curl virus（TYLCV）from Xinmin area of Liaoning province by PCR reaction,and then the generated specific segments were collected,cloned and sequenced.The results of Blast showed that the nucleotide sequence of 3samples had high identity from TYLCV.PCR detection for TYLCV was high sensitive,high efficient and with high specificity. |
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| Keywords: | tomato tomato yellow leaf curl virus molecular identification |
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