| 冷冻干燥对丝素蛋白凝胶结构的影响 |
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| 引用本文： | 刘明,闵思佳,朱良均.冷冻干燥对丝素蛋白凝胶结构的影响[J].蚕业科学,2007,33(2):246-249. |
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| 作者姓名： | 刘明 闵思佳 朱良均 |
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| 作者单位： | 浙江大学应用生物资源研究所,杭州,310029;浙江大学应用生物资源研究所,杭州,310029;浙江大学应用生物资源研究所,杭州,310029 |
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| 摘 要： | 为了探讨冷冻干燥对丝素蛋白凝胶结构的影响,利用傅里叶变换红外光谱(FTIR)研究了丝素凝胶在冷冻干燥前后二级结构的变化。发现在冷冻干燥过程中,丝素蛋白中分子链内β-折叠结构含量增加,分子链间β-折叠结构含量减少,并有部分无规卷曲结构转变为β-折叠结构;但冷冻干燥前后总的β-折叠结构含量变化不大。
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| 关 键 词： | 丝素蛋白凝胶 冷冻干燥 傅里叶变换红外光谱 链间β结构 |
| 文章编号： | 0257-4799(2007)02-0246-04 |
| 修稿时间： | 2006-06-13 |
Effect of Freeze-dry on the Structure of Silk Fibroin Gel |
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| LIU Ming,MIN Si-Jia,ZHU Liang-Jun.Effect of Freeze-dry on the Structure of Silk Fibroin Gel[J].Acta Sericologica Sinica,2007,33(2):246-249. |
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| Authors: | LIU Ming MIN Si-Jia ZHU Liang-Jun |
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| Institution: | Applied Bioresource Research Institute, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | In order inquire into the effect of freeze-dry on the structure of fibroin gel,the change of secondary structure of silk fibroin gel with or without freeze-dry was studied by FTIR.It has been found that the content of intramolecular β-sheet structure increased in the freeze-dry process,while the intermolecular β-sheet structure decreased.And part of random coils structure changed to β-sheet.However,in the whole process,the content of β-sheet structure changed a little. |
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| Keywords: | Silk fibroin gel Freeze-dry FTIR Intramolecular β structure |
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