| 转cry 1Iem基因大豆的培育及抗虫性检测 |
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| 引用本文： | 陈秀华,柏锡,潘欣,翟红,才华,纪巍,李勇,朱延明.转cry 1Iem基因大豆的培育及抗虫性检测[J].大豆科学,2009,28(6). |
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| 作者姓名： | 陈秀华 柏锡 潘欣 翟红 才华 纪巍 李勇 朱延明 |
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| 作者单位： | 东北农业大学生命科学学院植物生物工程研究室,黑龙江,哈尔滨,150030 |
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| 基金项目： | 国家高技术研究发展计划资助项目，转基因生物新品种培育重大专项资助项目，东北农业大学创新团队资助项目 |
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| 摘 要： | 以大豆子叶节为外植体,应用农杆菌介导法,将抗虫(cry1Iem)基因转化大豆.筛选标记为bar基因.经Glufasinate筛选,获得大量抗性植株.对转基因T_0、T_1、T_2代植株进行PCR检测,初步证明cry1Iem基因已经整合到大豆基因组中.对T2代PCR阳性植株幼嫩豆荚,采用圆盘分隔法接人初孵幼虫,进行初步的抗虫性检测,得到1株具有明显抗虫效果和7株抗虫效果较好的转基因植株.
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| 关 键 词： | 大豆 cry1Iem基因 子叶节 转基因抗性植株 抗虫性检测 |
Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay |
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| CHEN Xiu-hua,BAI Xi,PAN Xin,ZHAI Hong,CAI Hua,JI Wei,Li Yong,ZHU Yan-ming.Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay[J].Soybean Science,2009,28(6). |
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| Authors: | CHEN Xiu-hua BAI Xi PAN Xin ZHAI Hong CAI Hua JI Wei Li Yong ZHU Yan-ming |
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| Institution: | CHEN Xiu-hua,BAI Xi,PAN Xin,ZHAI Hong,CAI Hua,JI Wei,LI Yong,ZHU Yan-ming(Plant Bioengineering Laboratory,College of Life Sciences,Northeast Agriculture University,Harbin 150030,Heilongjiang,China) |
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| Abstract: | Contyledonary nodes were used as explants,and insect resistant gene cryllem was transformed into soybean via Agrobacterium-mediated method. Bar served as selection marker, and glufasinate was used in explants transformation to screen resistant shoots. Lots of resistant plants were obtained. With PCR detection of T_0、T_1、T_2 generation,insect resistant gene cry 1Iem was proved to be transformed into soybean preliminarily. Furthermore, immature pods of PCR positive plants in T_2 generation and newly hatched larvaes were introduced, and initial insect resistant assay was made by disk method. Result suggested that one transgenic plant had an obvious resistant effect,and another seven plants proved to have some effect. |
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| Keywords: | Soybean cryllem gene Contyledonary nodes Transformed resistant plants Insect resistant assay |
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