| 马占相思心腐病发生初期的病原鉴定 |
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| 引用本文： | 王勇,贺伟,黄烈健,陈敏.马占相思心腐病发生初期的病原鉴定[J].中国农学通报,2011,27(16):11-16. |
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| 作者姓名： | 王勇 贺伟 黄烈健 陈敏 |
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| 作者单位： | 1. 北京林业大学省部共建森林培育与保护教育部重点实验室,北京,100083
2. 中国林业科学院热带林业研究所,广州,510520 |
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| 基金项目： | 十一五科技支撑计划课题 |
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| 摘 要： | 为了明确马占相思心腐病发生初期的病原菌种类，为病害的早期诊断和防治提供依据，笔者从广东省龙眼洞国营林场外表无症状的6年生马占相思树上采集病样36份，分离出3种担子菌，通过田间枝条接种、室内木块接种测定其致病性。结果显示，3种担子菌在6年生马占相思树1年生健康枝条上接种20天，枝条髓心变褐深度分别为5.0、2.4、7.0 cm；室内25℃下接种61天，木块腐朽质量损失分别为25.88%、31.48%、21.02%。对3种担子菌进行PCR扩增，测定核糖体DNA（rDNA）内转录间隔区（ITS）的序列，将测序结果在GenBank中进行同源性比对分析，结合形态特征，确定3种病原菌分别为Phanerochaete avellanea、Peniophora aurantiaca、Phlebia brevispora。得出结论：Phanerochaete avellanea、Peniophora aurantiaca和Phlebia brevispora具有侵染枝条和使木块腐朽的能力，是马占相思心腐病发生初期的病原菌。
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| 关 键 词： | 石榴 石榴 分离 石榴酒酵母 发酵工艺 |
| 收稿时间： | 2011/3/23 0:00:00 |
| 修稿时间： | 2011/4/14 0:00:00 |
Pathogen Identification for Heart-rot of Acacia mangium in the Initial Stage |
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| Wang Yong,He Wei,Huang Liejian,Chen Min.Pathogen Identification for Heart-rot of Acacia mangium in the Initial Stage[J].Chinese Agricultural Science Bulletin,2011,27(16):11-16. |
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| Authors: | Wang Yong He Wei Huang Liejian Chen Min |
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| Institution: | Wang Yong 1,He Wei 1,Huang Liejian 2,Chen Min 1 (1 Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,2 Research Institute of Tropical Forestry,CAF,Guangzhou 510520) |
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| Abstract: | In order to clarify the pathogen species of heart rot of Acacia mangium in the initial stage and provide the basis for the early diagnosis and prevention of the disease,the author collected 36 diseased samples from asymptomatic 6-year-old living tree of Acacia mangium from a stand at Longyandong forest farm in Guangdong Province,from which 3 species of basidiacota fungi were isolated.The pathogenicity of 3 basidiacota fungi was tested through inoculation them to 1-year-old branch on 6-year-old living tree i... |
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| Keywords: | Acacia mangium heart rot pathogenicity pathogen identification |
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