| 蟾酥注射液对免疫抑制小鼠免疫功能的调节作用 |
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| 引用本文： | 郭维霄,仲伟婷,李文华,杨小丰,杨振国.蟾酥注射液对免疫抑制小鼠免疫功能的调节作用[J].中国农学通报,2011,27(14):45-49. |
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| 作者姓名： | 郭维霄 仲伟婷 李文华 杨小丰 杨振国 |
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| 作者单位： | 吉林大学畜牧兽医学院,长春,130062 |
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| 基金项目： | "十一五"国家科技支撑计划 |
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| 摘 要： | 摘要：本实验研究蟾酥注射液对免疫抑制小鼠免疫功能的调节作用。采用药理免疫学方法制造免疫低下的动物模型，测定脾脏重量并计算脾脏指数；ELISA方法检测IgG，IgG1，IgG2b抗体水平；MTT法检测脾淋巴细胞增殖反应；ELISA方法检测细胞因子（IFN-γ、IL-4、IL-10）水平。测定结果表明，蟾酥注射液能够增强免疫低下小鼠脾脏指数，提高抗体水平；在ConA或者LPS刺激下提高脾细胞增殖率，促进淋巴细胞分泌细胞因子，说明蟾酥注射液在一定剂量下能够缓解氟苯尼考引起的免疫抑制。
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| 关 键 词： | 紫花苜蓿 紫花苜蓿 离体再生体系 子叶 下胚轴 |
| 收稿时间： | 2010/12/15 0:00:00 |
| 修稿时间： | 4/8/2011 12:00:00 AM |
Regulative effects of Bufonis Parenteral on immunosuppression in mice |
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| Guo Weixiao,Zhong Weiting,Li Wenhua,Yang Xiaofeng,Yang Zhenguo.Regulative effects of Bufonis Parenteral on immunosuppression in mice[J].Chinese Agricultural Science Bulletin,2011,27(14):45-49. |
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| Authors: | Guo Weixiao Zhong Weiting Li Wenhua Yang Xiaofeng Yang Zhenguo |
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| Institution: | Guo Weixiao,Zhong Weiting,Li Wenhua,Yang Xiaofeng,Yang Zhenguo ( College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062) |
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| Abstract: | In the present study, regulative effects of bufonis parenteral sloution on-induced immunosuppression in mice were evaluated. Animal model of fl orfenicol-induced immunosuppression was made. Slpeens were weighted and calculated weight index. OVA-specific IgG, IgG 1 , IgG 2b were measured by ELISA, splenocytes were harvested for determining lymphocyte proliferation and spleen lymphocyte secrete cytokines were measured by ELISA method. The results showed that bufonis parenteral solution could increase the weig... |
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| Keywords: | florfenicol Bufonis Parenteral OVA immunosuppression cytokines |
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