| 孕酮人工抗原的合成及间接ElISA法鉴定 |
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| 引用本文： | 李志源,杨百亮,艾霞.孕酮人工抗原的合成及间接ElISA法鉴定[J].中国农学通报,2011,27(7):360-363. |
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| 作者姓名： | 李志源 杨百亮 艾霞 |
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| 作者单位： | 天津农学院动科系,天津,300384 |
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| 摘 要： | 摘要：本研究采用二环己基碳二亚胺法将孕酮衍生物P4-11α-半琥珀酸酯与载体BSA和OVA进行偶联，制备了孕酮人工完全抗原（免疫原和检测原），然后免疫Balb/c小鼠，制备孕酮多克隆抗体。用紫外光谱扫描法鉴定偶联是否成功并用间接Elisa法对孕酮多克隆抗体进行鉴定；结果显示，孕酮和BSA、OVA的偶联比分别为18:1、10:1，免疫血清孕酮抗体效价高达1：25600，这说明成功制备了免疫原和检测原，为孕酮胶体金试纸条的研制奠定了基础。
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| 关 键 词： | 稳定性 稳定性 |
| 收稿时间： | 2010/11/10 0:00:00 |
| 修稿时间： | 2010/12/11 0:00:00 |
Synthesis and Detection of Artificial Antigen for Progesterone |
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| Li Zhiyuan,Zhang Jianbin,Ai Xia,Yang Bailiang.Synthesis and Detection of Artificial Antigen for Progesterone[J].Chinese Agricultural Science Bulletin,2011,27(7):360-363. |
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| Authors: | Li Zhiyuan Zhang Jianbin Ai Xia Yang Bailiang |
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| Institution: | Li Zhiyuan,Zhang Jianbin,Ai Xia,Yang Bailiang (Department of Animal Science,Tianjin Agricultural University,Tianjin 300384) |
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| Abstract: | To develop a rapid technology for the detection of progesterone,Two kinds of artificial antigen for progesterone (P 4 ) were synthesized and identified in this research.Progesterone-11alpha-hemisuccinate was conjugated with carrier protein Albumin Bovine V(BSA) and Albumin egg (OVA) by dicyclohexylcarbodiimide (DCC).Then,10 Balb/c mice were injected with immunogen P 4 -BSA to produce antibody against P 4 .The prepared artificial antigens were accredited by ultraviolet scan and the titer of antibody against ... |
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| Keywords: | progesterone artificial antigen ultraviolet scan Elisa |
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