| 紫苏苯丙氨酸解氨酶基因片段克隆及序列分析 |
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| 引用本文： | 吕晓玲,孙雪梅,王芳,郝磊,孙晶磊.紫苏苯丙氨酸解氨酶基因片段克隆及序列分析[J].中国农学通报,2011,27(9):347-351. |
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| 作者姓名： | 吕晓玲 孙雪梅 王芳 郝磊 孙晶磊 |
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| 作者单位： | 天津科技大学,天津,300457 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 苯丙氨酸解氨酶（Phenylalanine ammonia-lyase，PAL）是迷迭香酸合成途径中苯丙氨酸支路的关键酶，它催化苯丙氨酸生成肉桂酸，完成该支路第一步反应。本实验利用同源克隆方法成功克隆了紫苏PAL基因cDNA片段，命名为PerPAL-1（GenBank登录号：HQ388347.1），该片段长399 bp，编码133个氨基酸。通过氨基酸序列比对分析，发现其氨基酸序列与丹参和藿香PAL该片段的同源性分别高达96%和95%。PAL系统进化树表明PerPAL-1与唇形科植物的PAL亲缘关系最近。PerPAL-1基因在叶中表达最强，根中次之，而在茎中表达最弱。
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| 关 键 词： | 两步法RT-PCR 两步法RT-PCR |
| 收稿时间： | 2010/12/28 0:00:00 |
| 修稿时间： | 2011/1/30 0:00:00 |
Molecular Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase Gene Fragment in Perilla frutescens |
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| Lv Xiaoling,Sun Xuemei,Wang Fang,Hao Lei,Sun Jinglei.Molecular Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase Gene Fragment in Perilla frutescens[J].Chinese Agricultural Science Bulletin,2011,27(9):347-351. |
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| Authors: | Lv Xiaoling Sun Xuemei Wang Fang Hao Lei Sun Jinglei |
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| Institution: | Lv Xiaoling,Sun Xuemei,Wang Fang,Hao Lei,Sun Jinglei(Tianjin University of Science and Technology,Tianjin 300457) |
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| Abstract: | Phenylalanine ammonia-lyase(PAL),responsible for catalyzing the conversion of phenylalanine to cinnamic acid to finish the first step of phenylalanine pathway,was the key enzyme during the biosynthesis of rosmarinic acid.The cDNA fragment of PAL gene was successfully cloned by homology cloning method(Accession No.HQ388347.1),399 bp and encoded 133 amino acids.It was designated as PerPAL-1.The results of amino acid sequence analysis showed that the identity of the sequence of PerPAL-1 amino acid with that of... |
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| Keywords: | Perilla frutescens phenylalanine ammonia-lyase gene cloning sequence analysis |
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