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| 甘肃金鳟AFLP体系建立及应用 | |
| 引用本文： | 苏军虎,张艳萍,魏彦明.甘肃金鳟AFLP体系建立及应用[J].中国农学通报,2011,27(7):400-404. |
| 作者姓名： | 苏军虎 张艳萍 魏彦明 |
| 作者单位： | 1. 甘肃农业大学生命科学技术学院,兰州,730070;甘肃省水产科学研究所甘肃省冷水性鱼类种质资源与遗传育种重点实验室,兰州,730000 2. 甘肃省水产科学研究所甘肃省冷水性鱼类种质资源与遗传育种重点实验室,兰州,730000;甘肃农业大学动物科学技术学院,兰州,730070 3. 甘肃省水产科学研究所甘肃省冷水性鱼类种质资源与遗传育种重点实验室,兰州,730000 4. 甘肃农业大学生命科学技术学院,兰州,730070 5. 甘肃农业大学动物科学技术学院,兰州,730070 6. 甘肃省水产科学研究所甘肃省冷水性鱼类种质资源与遗传育种重点实验室,兰州,730000;甘肃农业大学动物医学院,兰州,730070 |
| 基金项目： | 科技部成果转化项目，甘肃省自然科学基金 |
| 摘 要： | 甘肃金鳟是我国自主培育的虹鳟新品种，为进行其种质资源研究和遗传管理，以其尾鳍为试验材料,提取基因组DNA，对影响甘肃金鳟扩增片断长度多态性(AFLP)反应体系进行优化，包括模板DNA浓度、基因组酶切时间、选择性扩增中Mg2+、预扩增产物稀释倍数及选扩性引物M+3/E+3配比等进行比较分析，建立了适于甘肃金鳟的AFLP反应体系。即：100 ng 基因组DNA，3 U EcoR I 37℃酶切3 h，再 Mse I 65℃酶切5 h；然后用1 U的T4连接酶连接12 h, 选扩25 μl PCR反应体系中Mg2+2.0 mmol/L,预扩产物稀释30倍,选扩引物M+3/E+3配比为8∶1，所得产物经电泳和银染后可获得清晰条带，效果良好；筛选出了适宜甘肃金鳟品种分析的13对选择性引物。 |
| 关 键 词： | 番茄 番茄 色差 番茄红素 主基因+多基因遗传 |
| 收稿时间： | 2010/9/10 0:00:00 |
| 修稿时间： | 2010/10/29 0:00:00 |
Establishment and Application of AFLP Reaction System in Gansu Rainbow Trout |
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| Su Junhu,Zhang Yanping,Lou Zhongyu,Jiao Wenlong,Wang Tai,Yang Jianbao,Wu Jianping,Wei Yanming.Establishment and Application of AFLP Reaction System in Gansu Rainbow Trout[J].Chinese Agricultural Science Bulletin,2011,27(7):400-404. | |
| Authors: | Su Junhu Zhang Yanping Lou Zhongyu Jiao Wenlong Wang Tai Yang Jianbao Wu Jianping Wei Yanming |
| Institution: | Su Junhu 1,2,Zhang Yanping 2,3,Lou Zhongyu 2,Jiao Wenlong 2,Wang Tai 2,Yang Jianbao 1,Wu Jianping 3,Wei Yanming 2,4 (1 College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,2 Gansu Fisheries and Science Research Institute,Gansu Key Laboratory of Cold Water Fishes Germplasm Resources and Genetics Breeding,Lanzhou 730000,3 A nimal Science and Technology,4 College of Veterinary Medicine,Lanzhou 730070 ) |
| Abstract: | Oncorhynchus mykiss is an important reared freshwater species,which has been cultured for more than thirty years in Gansu Province,China.Gansu golden trout is a new breed developed in Gansu,which had pass the National Aquaculture Species Identification Committee identifying and was recommend as the species reared in the northwestern areas.In this study,the DNA was extracted from the caudal fin of Gansu rainbow trout,and the conditions for AFLP analysis were optimized,including DNA concentration,dNTPs concen... |
| Keywords: | AFLP Gansu Rainbow Trout reaction system establishment optimization |
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