| 岩栖蝮蛇毒纤溶酶基因克隆、序列分析及真核表达载体的构建与鉴定 |
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| 引用本文： | 于德涵,赵文阁,王广慧,李贺.岩栖蝮蛇毒纤溶酶基因克隆、序列分析及真核表达载体的构建与鉴定[J].中国农学通报,2011,27(14):17-21. |
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| 作者姓名： | 于德涵 赵文阁 王广慧 李贺 |
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| 作者单位： | 1. 绥化学院生物与食品工程系,黑龙江绥化,152061
2. 哈尔滨师范大学生命科学与技术学院,哈尔滨,150025 |
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| 摘 要： | 克隆岩栖蝮蛇(Gloydius saxatilis)纤维溶解酶(Fibrinolytic enzyme,FLE)基因,分析基因序列并对该基因的功能进行分析;构建真核表达载体并鉴定.从岩栖蝮蛇毒腺细胞中提取总RNA,通过RT-PCR法扩增纤溶酶基因,与pMDl8-T载体连接进行TA克隆,再进行序列的测定并且对该序列进行分...
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| 关 键 词： | 岩栖蝮蛇 纤溶酶 基因克隆 序列分析 真核表达载体 |
| 收稿时间： | 2/9/2011 12:00:00 AM |
| 修稿时间： | 2011/3/16 0:00:00 |
Cloning and Sequence Analysis, Construction and Indentification of Eukaryotic Expression Vector of Fibrinolytic Enzyme from Gloydius saxatilis |
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| Yu Dehan,Zhao Wenge,Wang Guanghui,Li he.Cloning and Sequence Analysis, Construction and Indentification of Eukaryotic Expression Vector of Fibrinolytic Enzyme from Gloydius saxatilis[J].Chinese Agricultural Science Bulletin,2011,27(14):17-21. |
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| Authors: | Yu Dehan Zhao Wenge Wang Guanghui Li he |
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| Institution: | Yu Dehan 1,Zhao Wenge 2,Wang Guanghui 1,Li he 1 ( 1 Department of Biotechnology and Food Engineering of Suihua College,Suihua Heilongjiang 152061,2 College of Life Science and Technology in Harbin Normal University,Harbin 150025) |
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| Abstract: | Fibrinolytic enzyme gene of Gloydius saxatilis was cloned, then analysis the gene sequence and function; construction and indentification of eukaryotic expression vector of FLE gene. Total RNA was extracted from venom cells from Gloydius saxatilis, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the gene sequence. The FLE gene was inserted into the vector pEGFP-C1. This recombinant plasmid pEGFP-FLE was identified by digestion of endo... |
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| Keywords: | Gloydius intermedius fibrinolytic enzyme gene cloning sequence analysis eukaryotic expression vector |
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