| 玫瑰RdGASA4-like基因启动子的分离及其在甘蓝中的瞬时表达分析 |
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| 引用本文： | 张强,张晓伟,孟月娥,李艳敏,王慧娟,王利民.玫瑰RdGASA4-like基因启动子的分离及其在甘蓝中的瞬时表达分析[J].中国农学通报,2011,27(8):123-127. |
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| 作者姓名： | 张强 张晓伟 孟月娥 李艳敏 王慧娟 王利民 |
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| 作者单位： | 河南省农业科学院园艺研究所,郑州,450002 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目 |
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| 摘 要： | 在前期的研究中，一个玫瑰RdGASA4-like基因的cDNA和DNA序列全长利用同源克隆结合RACE的方法首次得到克隆。为深入了解该基因在赤霉素调控植物发育过程中的作用，通过TAIL-PCR的方法首次克隆该基因起始密码子ATG上游685bp的5’-UTR和部分启动子序列，在启动子序列分析的基础上，构建其与GUS基因融合表达载体，命名为pBI-GP685。通过农杆菌介导的方法对甘蓝外植体子叶-子叶柄进行启动子瞬时表达研究，结果表明分离得到的启动子具有启动报告基因GUS表达的活性。
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| 关 键 词： | RdGASA4-like 分离 启动子 瞬时表达 |
| 收稿时间： | 2010/7/23 0:00:00 |
| 修稿时间： | 2010/8/27 0:00:00 |
Isolation and Transient Expression Analysis in Cabbage of the Promoter of RdGASA4-like from Rosa damascene |
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| Zhang Qiang,Zhang Xiaowei,Meng Yue'e,Li Yanmin,Wang Huijuan,Wang Limin.Isolation and Transient Expression Analysis in Cabbage of the Promoter of RdGASA4-like from Rosa damascene[J].Chinese Agricultural Science Bulletin,2011,27(8):123-127. |
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| Authors: | Zhang Qiang Zhang Xiaowei Meng Yue'e Li Yanmin Wang Huijuan Wang Limin |
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| Institution: | (Institute of Horticulture Science,Henan Academy of Agriculture Science,Zhengzhou 450002) |
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| Abstract: | In previous study,the full length DNA and cDNA of theRdGASA 4-like gene had been isolated from Damask rose（Rosa damascena ）through homology cloning combined with rapid amplification of cDNA ends （RACE）.In order to further understand the role ofRdGASA 4-like in the regulating network on plant development of gibberellic acid,its 685 bp 5’-UTR and partial sequence upstream ATG ofRdGASA 4-like was obtained by Thermal Asymmetric Interlaced Polymerase Chain Reaction （TAIL-PCR）.Based on sequence analysis,it was fused to a GUS gene to construct plant expression vector,named as pBI-GP685.By agrobacterium-mediated method,the explants of petiole with cotyledon of cabbage were transformed.The transient expression study showed that the obtained promoter ofRdGASA 4-like was active and could drive the activity of GUS. |
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| Keywords: | RdGASA 4-like isolation promoter transient expression |
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