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基于RAPD标记与形态标记的辣椒自交系间亲缘关系的比较研究
引用本文:罗英,吴立东,曾绍贵,朱邦彤,许旭明,温庆放.基于RAPD标记与形态标记的辣椒自交系间亲缘关系的比较研究[J].中国农学通报,2011,27(16):136-141.
作者姓名:罗英  吴立东  曾绍贵  朱邦彤  许旭明  温庆放
作者单位:1. 三明市农业科学研究所,福建沙县,365509
2. 福建省农业科学院作物研究所,福州,350013
摘    要:为了更加准确地测定辣椒亲本间的亲缘关系,提高辣椒育种效率。利用RAPD标记技术对60份辣椒自交系进行了基因组多态性分析,并将形态学聚类和分子鉴定加以对比。结果表明:(1)对60个辣椒自交系的12个数量性状进行了聚类分析,遗传距离在4.8时,将其分为5类。(2)利用RAPD标记对60份辣椒自交系进行了基因组多态性分析,遗传距离D值在0.35处,将辣椒的3个变种基本分开。(3)RAPD标记计算的遗传相异系数矩阵和形态学计算的遗传距离矩阵做相关性分析,其相关系数为0.26,达到极显著。因此,在研究辣椒自交系亲缘关系时,可将两者相结合进行遗传相似性的分析,提高其准确性。

关 键 词:遗传多样性  遗传多样性  
收稿时间:2011/1/10 0:00:00
修稿时间:2011/2/28 0:00:00

Study on pepper inbred lines′ relationship based on RAPD technique and morphological clustering
Luo Ying,Wu Lidong,Zeng Shaogui,Zhu Bangtong,Xu Xuming,Wen Qingfang.Study on pepper inbred lines′ relationship based on RAPD technique and morphological clustering[J].Chinese Agricultural Science Bulletin,2011,27(16):136-141.
Authors:Luo Ying  Wu Lidong  Zeng Shaogui  Zhu Bangtong  Xu Xuming  Wen Qingfang
Institution:Luo Ying 1,Wu Lidong 1,Zeng Shaogui 1,Zhu Bangtong 1,Xu Xuming 1,Wen Qingfang 2 (1 Sanming Institute of Agricultural Sciences,Shaxian Fujian 365509,2 Crop Institute Fujian Academy of Agricultural Sciences,Fuzhou 350013)
Abstract:To more accurately determine the relationships between pepper parents and improve the pepper breeding efficiency,60 pepper inbred lines were used to analyze genomic polymorphism by RAPD and morphological clustering.The results of morphological clustering with molecular identification were compared too.The results showed:(1) 60 pepper inbred lines were classified into five groups at the genetic distance level of 4.8 that were calculated by 12 agronomic quantitative traits.(2) 60 pepper inbred lines were clas...
Keywords:pepper  RAPD  morphological analysis  genetic relationships  
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