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| 菠萝蜜的染色体制片优化及核型分析 | |
| 引用本文： | 劳世辉,魏岳荣,盛鸥,梁国鲁.菠萝蜜的染色体制片优化及核型分析[J].中国农学通报,2011,27(16):215-219. |
| 作者姓名： | 劳世辉 魏岳荣 盛鸥 梁国鲁 |
| 作者单位： | 1. 西南大学园艺园林学院,重庆400715;广东省农业科学院果树研究所,广州510640 2. 广东省农业科学院果树研究所,广州,510640 3. 西南大学园艺园林学院,重庆,400715 |
| 基金项目： | 国家科技支撑项目;科技部国际合作项目 |
| 摘 要： | 应用改良的去壁低渗法研究比较了不同预处理剂对菠萝蜜染色体制片的效果及其染色体数目和核型。结果表明：以0.002 mol/L 8-羟基喹啉与0.2%秋水仙素（1:1）混合液作为预处理剂，预处理3 h效果较好。菠萝蜜的染色体数目为2n=2x=56，为二倍体，主要由中部和近中部着丝点染色体构成，9号染色体上附着一对随体；最长染色体/最短染色体为2.08:1，臂比大于2的染色体占全组染色体的3.5%，属2A核型，核型公式为2n=2M+50m（2SAT）+4sm。该文首次发现菠萝蜜可能存在B染色体。 |
| 关 键 词： | 遗传多样性 遗传多样性 |
| 收稿时间： | 2011/3/24 0:00:00 |
| 修稿时间： | 2011/5/20 0:00:00 |
Optimization of Chromosome Mounting Technique and Karyotype Analysis of Jackfruit (Artocarpus heterophyllus Lam.) |
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| Lao Shihui,Wei Yuerong,Sheng Ou,Liang Guolu.Optimization of Chromosome Mounting Technique and Karyotype Analysis of Jackfruit (Artocarpus heterophyllus Lam.)[J].Chinese Agricultural Science Bulletin,2011,27(16):215-219. | |
| Authors: | Lao Shihui Wei Yuerong Sheng Ou Liang Guolu |
| Institution: | Lao Shihui 1,2,Wei Yuerong 2,Sheng Ou 2,Liang Guolu 1 (1 College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,2 Fruit Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640) |
| Abstract: | In this paper,the chromosome number and karyotype of jackfruit was studied after detaching the cell wall and treated with low osmotic solution,and the effects of different pretreatments were also investigated.The results showed that the optimum pretreatment chemical was the mixture of 0.2% colchicine and 0.002 mol/L 8-hydroxyquinoline (1:1),with the pretreatment time for 3 hours.The karyotype analysis showed that jackfruit was diploid (2n=2x=56).The ratio of the longest chromosome to the shortest one was 2.... |
| Keywords: | jackfruit chromosome preparation B chromosome karyotype |
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