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| 地黄实时定量PCR内参基因的筛选 | |
| 引用本文： | 侯维海,孙鹏,陈全家,李先恩.地黄实时定量PCR内参基因的筛选[J].中国农学通报,2011,27(17):76-82. |
| 作者姓名： | 侯维海 孙鹏 陈全家 李先恩 |
| 作者单位： | 1. 新疆农业大学农学院,乌鲁木齐830052;中国医学科学院中国协和医科大学药用植物研究所,北京100193 2. 中国医学科学院中国协和医科大学药用植物研究所,北京,100193 3. 新疆农业大学农学院,乌鲁木齐,830052 |
| 摘 要： | 本文通过RT-qPCR分析了地黄中7个传统内参基因18S、EF-1?、ACT11、UBQ10、UBQ5、TUB5、GAPDH和4个新报道的内参基因PP2A、RPⅡ、HSP90、TIP41的mRNA表达差异情况，分别利用Ct值比较，GeNorm、NormFiner和BestKeeper软件分析它们在两个发育时期、八种不同组织器官中表达稳定性。结果表明：在地黄花器官中（花瓣、花托、雄蕊、雌蕊、子房），TIP41和UBQ10表达稳定；在地黄生殖生长期和营养生长期不同器官中（根、茎、叶），TIP41和UBQ5表达稳定。因此，以上两组基因分别适宜作为不同营养器官的内参基因。 |
| 关 键 词： | 再生植株 再生植株 |
| 收稿时间： | 2010/11/8 0:00:00 |
| 修稿时间： | 1/6/2011 12:00:00 AM |
Selection of the Reference Genes for Gene Expression Studies in Rehmannia glutinosa by Real-time Quantitative PCR |
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| Hou Weihai,Sun Peng,Chen Quanjia,Li Xian'en.Selection of the Reference Genes for Gene Expression Studies in Rehmannia glutinosa by Real-time Quantitative PCR[J].Chinese Agricultural Science Bulletin,2011,27(17):76-82. | |
| Authors: | Hou Weihai Sun Peng Chen Quanjia Li Xian'en |
| Institution: | Hou Weihai, Sun Peng, Chen Quanjia, Li Xian’en (1College of Agronomy, Xinjiang Agricultural University, Urumqi 830052; 2Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences and Peking Union Medical College, Beijing 100193) |
| Abstract: | A total of 11 reference genes were systematically compared in different tissues of Rehmannia glutinosa using RT-qPCR, including 7 traditional reference genes （ACT 11, 18S , TUB 5, GAPDH , EF- 1a , UBQ 10 andUBQ 5） and 4 novel candidates reference gene （HSP 90,PP 2A ,RPⅡ andTIP 41） in this study. The mRNA levels of these genes were analyzed among the organs of flowers, roots, stems and leaves representing the vegetative growth phase and the generative growth phase. The stabilities of the 11 candidate reference genes were ranked by the Ct value comparison and three softwares （GeNorm, NormFiner and BestKeeper）. The results showed that the expression ofTIP 41 andUBQ 10 was stable in different tissues of sepals, petals, stamens, pistils, and ovary from flower. For the case of the roots, stems and leaves sampled in vegetative growth phase and generative growth phase,UBQ 5 andTIP 41 were expressed stably. As a result, the 2 groups of candidate genes were selected as reference genes forRehmannia glutinosa. |
| Keywords: | real-time quantitative PCR reference gene GeNorm Rehmannia glutinos |
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