| 高效纤维素降解菌短小芽孢杆菌（Bacillus pumilus）T-7的筛选、鉴定及降解能力的研究 |
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| 引用本文： | 张立静,李术娜,朱宝成.高效纤维素降解菌短小芽孢杆菌（Bacillus pumilus）T-7的筛选、鉴定及降解能力的研究[J].中国农学通报,2011,27(7):112-118. |
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| 作者姓名： | 张立静 李术娜 朱宝成 |
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| 作者单位： | 河北农业大学生命科学学院,河北保定,071001 |
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| 摘 要： | 为了得到一株具有降解纤维素性能的产芽孢菌株，采用加热富集芽孢菌及刚果红脱色圈的初筛方法，从菜地土壤、动物粪便、青贮饲料等样品中分离筛选出41株能够降解纤维素的产芽孢细菌。对初筛菌株发酵培养，测定发酵液透明圈直径及纤维素酶活力，菌株T-7具有显著的降解能力，纤维素酶活力达1678.89U/mL。通过形态观察鉴定、生理生化实验和16SrDNA序列分析对其进行种属鉴定，鉴定T-7菌株为短小芽孢杆菌（Bacillus pumilus）。研究了供试菌株T-7的降解工艺，获得了菌株发挥最大降解特性所需的最佳培养条件。结果表明，将菌株T-7以10亿活菌/1Kg的接种量接入玉米秸秆，并且添加辅助碳氮源2%蔗糖+2%尿素时，在发酵8天后对秸秆中纤维素的降解率达40.34%。研究结果为纤维素的生物降解发掘了新的菌种资源，并为秸秆的大规模降解利用奠定了基础。
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| 关 键 词： | 印度 咖啡 产业 印度 咖啡 产业 |
| 收稿时间： | 2010/9/13 0:00:00 |
| 修稿时间： | 2010/10/16 0:00:00 |
Screening,Identification and Degradation Conditions of Cellulose Decomposing Bacteria Bacillus pumilus T-7 |
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| Zhang Lijing,Li Shuna,Zhu Baocheng.Screening,Identification and Degradation Conditions of Cellulose Decomposing Bacteria Bacillus pumilus T-7[J].Chinese Agricultural Science Bulletin,2011,27(7):112-118. |
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| Authors: | Zhang Lijing Li Shuna Zhu Baocheng |
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| Institution: | Zhang Lijing,Li Shuna,Zhu Baocheng (College of Life Science,Agriculture University of Hebei,Baoding Hebei 071001) |
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| Abstract: | In order to screen strain degraded the cellulose,and obtain the best cultivation condition which made full use of degrading.More than 41 strains were isolated from vegetable field,animal manure and silage feed by heating gathering spore bacteria strains and Congo red culture medium transparent hydrolysis circle,and tested the cellulose strains through the method of DNS.The T-7 strain has the highest capability,its enzyme activity was 1678.89 U/mL.The mycelium shape and the bacterial colony,physiological and... |
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| Keywords: | spore cellulose screening identification degradation conditions |
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