| 土壤放线菌X1的筛选及其抑菌活性初探 |
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| 引用本文： | 徐路明 郝明亮 王金果 王桂芳 罗,兰.土壤放线菌X1的筛选及其抑菌活性初探[J].中国农学通报,2011,27(9):195-200. |
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| 作者姓名： | 徐路明 郝明亮 王金果 王桂芳 罗 兰 |
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| 作者单位： | 青岛农业大学农学与植物保护学院,山东青岛,266109 |
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| 摘 要： | 筛选具有抑菌活性的放线菌是开发农用活性抗生素的基础。本文采用生长速率法和孢子萌发法，测定了从土壤中分离出的6株拮抗放线菌的抑菌活性，并对抑菌活性较好的放线菌X1菌株进行初步研究。生长速率法试验表明，放线菌X1菌株发酵液对供试12种植物病原菌菌丝生长均有不同程度的抑制作用，发酵液对苹果腐烂病菌、苹果轮纹病菌和葡萄白腐病菌菌丝的抑制率均为100%。孢子萌发试验表明，放线菌X1菌株发酵液对黄瓜枯萎病菌孢子萌发的抑制率为100%。在浓度为1 mg/mL时，放线菌X1菌株发酵液乙酸乙酯萃取物对苹果腐烂病菌、苹果轮纹病菌和葡萄白腐病菌菌丝的抑菌率均大于90%。放线菌X1菌株抑菌谱较广，对植物病菌的防治有很大的研究开发潜力。
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| 关 键 词： | 化肥 化肥 |
| 收稿时间： | 2011/1/10 0:00:00 |
| 修稿时间： | 2011/1/29 0:00:00 |
The Study on Antifungal Activity and Screening of Actinomycete X1 Strain from Soil |
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| Xu Luming,Hao Mingliang,Wang Jinguo,Wang Guifang,Luo Lan.The Study on Antifungal Activity and Screening of Actinomycete X1 Strain from Soil[J].Chinese Agricultural Science Bulletin,2011,27(9):195-200. |
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| Authors: | Xu Luming Hao Mingliang Wang Jinguo Wang Guifang Luo Lan |
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| Institution: | Xu Luming,Hao Mingliang,Wang Jinguo,Wang Guifang,Luo Lan(College of Agronomy and Plant Protection,Qingdao Agricultural University,Qingdao Shandong 266109) |
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| Abstract: | Screening antifungal actinomycetes are foundation of agricultural antibiotics exploitation.The antifungal activity of 6 strains actinomycetes from soil were studied by mycelium growth rate,spore germination test.The antifungal activities of different extracts of actinomycete X1 strain were studied.The results of mycelium growth rate showed that the X1 strain displayed a broad spectrum inhibitory activity to all the tested 12 plant pathogenic fungi,of which the inhibition rate against 3 pathogens were 100%,s... |
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| Keywords: | antagonistic actinomycete screen fermentation antifungal activity |
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