| 响应面法优化解脂耶罗维亚酵母ypy01淀粉酶发酵条件研究 |
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| 引用本文： | 柳志强,李晓宇,张横江,刘美珍.响应面法优化解脂耶罗维亚酵母ypy01淀粉酶发酵条件研究[J].中国农学通报,2011,27(17):98-102. |
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| 作者姓名： | 柳志强 李晓宇 张横江 刘美珍 |
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| 作者单位： | 海南大学环境与植物保护学院,海南儋州,571737 |
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| 基金项目： | 海南省自然科学基金，海南大学科研启动基金 |
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| 摘 要： | 利用Plackett–Burman（PB）设计和响应面法对解脂耶罗维亚酵母ypy01淀粉酶生产条件进行了优化。PB设计筛选到3个显著因子，分别为可溶性淀粉浓度、蛋白胨浓度和初始pH。采用响应面法对3个显著因子进一步优化，获得了最佳培养基组成：0.84%可溶性淀粉，2.25%蛋白胨，1%酵母粉，3%NaCl，0.05% MgSO4，0.05% CaCl2，初始pH为6.8，在最优条件下，淀粉酶产量达到2012.50 U/mL。
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| 关 键 词： | POD POD |
| 收稿时间： | 2010/11/9 0:00:00 |
| 修稿时间： | 1/5/2011 12:00:00 AM |
Optimization of Amylase Production from Yarrowia lipolytica ypy01 by Using Response Surface Methodology |
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| Liu Zhiqiang,Tian Fu,Li Xiaoyu,Zhang Hengjiang,Liu Meizhen.Optimization of Amylase Production from Yarrowia lipolytica ypy01 by Using Response Surface Methodology[J].Chinese Agricultural Science Bulletin,2011,27(17):98-102. |
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| Authors: | Liu Zhiqiang Tian Fu Li Xiaoyu Zhang Hengjiang Liu Meizhen |
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| Institution: | Liu Zhiqiang,Tian Fu,Li Xiaoyu,Zhang Hengjiang,Liu Meizhen (College of Environment and Plant Protection,Hainan University,Danzhou Hainan 571737) |
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| Abstract: | The aim of this work was to optimize the operational parameters of marine yeastYarrowia lipolytica ypy01 to increase amylase production using Plackett-Burman (PB) design and response surface methodology (RSM). The results of PB design showed that the concentrations of starch and peptone and initial pH were the most significant factors to influence amylase production. A Central Composite Design (CCD), which is the standard design of RSM, was then employed to further optimize these three factors. The experime... |
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| Keywords: | Yarrowia lipolytica amylase optimization Placket-Burman design response surface methodology |
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