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| 内源激素对水稻不同外植体培养力的影响 | |
| 引用本文： | 王秀红,史向远,吴先军,汪旭东,周开达.内源激素对水稻不同外植体培养力的影响[J].中国农业科学,2004,37(12):1819-1823. |
| 作者姓名： | 王秀红 史向远 吴先军 汪旭东 周开达 |
| 作者单位： | 1. 四川农业大学水稻研究所,温江,611130;山西省农科院农业科技园区,太原,030031 2. 山西省农科院农业科技园区,太原,030031 3. 四川农业大学水稻研究所,温江,611130 |
| 基金项目： | 四川省青年基金资助项目 |
| 摘 要： | 利用HPLC法对愈伤组织诱导率和绿苗分化率有差异的几个水稻品种的不同外植体(花药、幼穗、幼胚和成熟胚)及其愈伤组织的内源激素含量进行测定分析。结果表明,内源激素的含量和配比是影响愈伤组织诱导和绿苗分化的关键因素之一。不同外植体中的内源激素对愈伤组织诱导率的表现为:Z ZR呈负效应,IAA呈正效应,GA除成熟胚外呈负效应。愈伤组织中内源激素对绿苗分化率的影响表现为:IAA和GA呈负效应,ABA、Z ZR呈正效应。内源激素的配比对愈伤组织诱导率和绿苗分化率有一定作用,表现为:IAA/Z ZR对愈伤组织诱导率有一定的正效应,Z ZR/IAA、ABA/IAA对绿苗分化率呈显著正相关。 |
| 关 键 词： | 水稻 内源激素 外植体 愈伤组织诱导率 绿苗分化率 |
| 收稿时间： | 2003-11-17 |
The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice |
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| WANG Xiu-hong,SHI Xiang-yuan,WU Xian-jun,WANG Xu-dong,ZHOU Kai-da.The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice[J].Scientia Agricultura Sinica,2004,37(12):1819-1823. | |
| Authors: | WANG Xiu-hong SHI Xiang-yuan WU Xian-jun WANG Xu-dong ZHOU Kai-da |
| Institution: | WANG Xiu-hong1,2,SHI Xiang-yuan2,WU Xian-jun1,WANG Xu-dong1,ZHOU Kai-da 1 |
| Abstract: | The endogenous hormone contents of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capabilities were analyzed by means of HPLC. The results showed that the contents and mixture ratio of endogenous hormones were one of the key factors affecting CIF (callus induction frequencies) and GPDF (green plantlet differentiation frequencies). The influence of endogenous hormones of different explants on CIF represented as: ZR showed negative effect, IAA did positive effect, and GA did negative effect except for mature embryos. The influence of endogenous hormones on GPDF showed: IAA and GA were negative effect, ABA and Z ZR were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z ZR had a positive effect on CIF, and there was a notable positive correlation between Z ZR/IAA and GPDF, so was ABA/IAA and GPDF. |
| Keywords: | Rice Endogenous hormone Explant Callus induction frequency Green plantlet differentiation frequency |
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