| 白玉凤尾蕨孢子繁殖技术的研究 |
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| 引用本文： | 徐艳,石雷,刘燕,李东.白玉凤尾蕨孢子繁殖技术的研究[J].园艺学报,2005,32(4):658-662. |
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| 作者姓名： | 徐艳 石雷 刘燕 李东 |
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| 作者单位： | (中国科学院植物研究所, 北京100093; 北京林业大学园林学院, 北京100083) |
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| 基金项目： | 科技部国家科技基础条件平台工作项目(2004DKA30430)，中国科学院农办项目(NK十五-C-13) |
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| 摘 要： | 研究了白玉凤尾蕨( Pteris cretica ‘Albo-lineata’) 孢子的无菌培养和常规繁殖方法。结果表明: 孢子萌发为书带蕨型, 配子体发育为水蕨型。1 /2MS培养基中萌发率最高, 可达82.3%; 低于2%的蔗糖浓度较适于孢子的萌发; MS培养基中配子体成苗率最高, 可达53.4%; 试管苗移栽成活率可达80%以上。草炭土+河沙(体积比1∶1) 是播种孢子的理想基质, 配子体成苗率达8912%; 当幼孢子体苗的第1片子叶长至1～2 cm长时分苗, 移栽到草炭土+蛭石+素砂+有机肥(体积比4∶2∶2∶1) 的栽培基质上,移栽成活率最高, 可达98.1%。幼孢子体苗长至10 cm左右时已经具有一定的观赏价值。
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| 关 键 词： | 凤尾蕨 孢子 无菌培养 常规繁殖 |
| 文章编号： | 0513-353X（2005）04-0658-05 |
| 收稿时间： | 2004-09-27 |
| 修稿时间： | 2004-09-272004-11-15 |
Studies on Spore Propagation of Pteris cretica 'Albo-lineata' |
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| Xu Yan,Shi Lei,Liu Yan,Li Dong.Studies on Spore Propagation of Pteris cretica ''''Albo-lineata''''[J].Acta Horticulturae Sinica,2005,32(4):658-662. |
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| Authors: | Xu Yan Shi Lei Liu Yan Li Dong |
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| Institution: | ( Institute of Botany, the Chinese Academy of Science, Beijng 100093, China; College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China) |
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| Abstract: | The spore sterile culture and routine propagation of Pteris cretica'Albo-lineata'were studied respectively.The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type.The maximum germination rate was obtained in 1/2MS medium,which reached 82.3%;the medium with sucrose concentration being less than 2% was appropriate for spore germination;the highest rate of gametophyte to sporophyte was achieved in MS medium,which reached 53.4%;the transplant survival rate of sporophyte in vitro reached above 80%.The mixed soil(the turf and the sand blend in the volume ratio of 1 to 1) was the optimal growing medium for spore routine propagation,in which the rate of gametophyte to the sporophyte was up to 89.2%.When the first cotyledon of the sporophyte seedling grew to 1-2 cm,the sporophyte seedlings should be replanted in the growing medium which was composed of turf,vermiculite,sand and organic fertilizers.The highest survival rate reached 98.1%.The young gametophyte seedlings of 10 cm height had already possessed ornamental values. |
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| Keywords: | Pteris cretica Spore Routine propagation Sterile culture |
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