| 韩国型鸭肝炎病毒福建株的分离与鉴定 |
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| 引用本文： | 施少华,程龙飞,江斌,万春和,傅光华,陈红梅,林芳,林建生,苏敬良,黄瑜.韩国型鸭肝炎病毒福建株的分离与鉴定[J].畜牧与兽医,2010,42(12). |
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| 作者姓名： | 施少华 程龙飞 江斌 万春和 傅光华 陈红梅 林芳 林建生 苏敬良 黄瑜 |
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| 摘 要： | 从福建省某养鸭场罹患鸭肝炎的病死鸭中分离到1株大小35～45 nm、十二面体对称的无囊膜病毒(暂命名为FJ01株),其鸭胚半数致死量(ELD50)为10~(-2.69)/0.2 mL,该病毒可被特异性韩国型鸭肝炎病毒免疫血清所中和,可被韩国型鸭肝炎病毒特异引物所扩增,而不能被1型鸭肝炎病毒引物所扩增。遗传进化分析表明鸭肝炎病毒FJ01株与韩国型鸭肝炎病毒关系密切,它们在进化树中共处一群。
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| 关 键 词： | 鸭肝炎病毒 韩国型 分离 鉴定 |
Isolation and identification of a South Korea-type duck hepatitis virus in Fujian province |
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| SHI Shao-hua,CHENG Long-fei,JIANG Bin,WAN Chun-he,FU Guang-hua,CHEN Hong-mei,LIN Fang,LIN Jian-sheng,SU Jing-liang,HUANG Yu.Isolation and identification of a South Korea-type duck hepatitis virus in Fujian province[J].Animal Husbandry & Veterinary Medicine,2010,42(12). |
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| Authors: | SHI Shao-hua CHENG Long-fei JIANG Bin WAN Chun-he FU Guang-hua CHEN Hong-mei LIN Fang LIN Jian-sheng SU Jing-liang HUANG Yu |
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| Abstract: | A virus strain, designated as FJO1, was isolated from a dead duckling characterized by duck hepatitis in Fujian province. Virus particles were non-enveloped, icosahedral symmetry, and with a diameter of 35～45 nm. The 50% embryo lethal dose (ELD50) in FJO1 was determined to be 10~(-2.69)/0. 2 mL with 10-day-old duck embryos. The virus could be neutralized by serum against South Korea-type of duck hepatitis virus (DHV). A pair of primers to South Korea-type DHV could be used to amplify the specific fragment from the genome of DHV FJO1 by RT-PCR, but the specific primers to DHV type 1 could not. Phylogenetic analysis showed that DHV FJO1 was close to South Korea-type DHV and they could be subdivided into a same cluster. |
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| Keywords: | duck hepatitis virus South Korea-type isolation identification |
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