| 中华鳖爆发性传染病研究——Ⅳ.不同生化类型嗜水气单胞菌aer基因的PCR比较 |
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| 引用本文： | 宋铁英,包晓东,郑伟文.中华鳖爆发性传染病研究——Ⅳ.不同生化类型嗜水气单胞菌aer基因的PCR比较[J].江西农业大学学报,2002,24(2):164-167. |
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| 作者姓名： | 宋铁英 包晓东 郑伟文 |
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| 作者单位： | 福建省农科院,生物技术中心,福建,福州,350003 |
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| 基金项目： | 福建省自然科学基金资助项目 (B0 0 10 0 2 6 ) |
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| 摘 要： | 用PCR技术对 5 2株不同来源和不同毒力的鳖源嗜水气单胞菌的aer基因进行了比较和分析。结果表明 ,b、d、e和 g四种生化型的嗜水气单胞菌含有特异的 2 0 9bp片段 ,而c和f两种生化型和其他鳖源致病菌却呈阴性反应 ,显示并非所有的嗜水气单孢菌都有aer基因 (产生气溶素 )。
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| 关 键 词： | 嗜水气单胞菌 aer基因 PCR |
| 文章编号： | 1000-2286(2002)02-0164-04 |
| 修稿时间： | 2001年12月3日 |
A Study on Outbreaks of Infectious Diseases in Soft-shell Turtles Ⅳ. Comparison of PCR result on aer gene with different types of biochemical reaction of Aeromonas hydrophlla |
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| SONG Tie-ying,BAO Xiao-dong,ZHENG Wei-wen.A Study on Outbreaks of Infectious Diseases in Soft-shell Turtles Ⅳ. Comparison of PCR result on aer gene with different types of biochemical reaction of Aeromonas hydrophlla[J].Acta Agriculturae Universitis Jiangxiensis,2002,24(2):164-167. |
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| Authors: | SONG Tie-ying BAO Xiao-dong ZHENG Wei-wen |
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| Abstract: | PCR technique was used to analyse and compare aer gene of 52 strains of Aeromonas hydrophlla from different sources and with different toxicity which were isolated from moribund soft-shell turtles. The results showed that four biochemical reaction types (types b, d, e and g) of the strains had a special 209bp fragment. However, types c, f and other pathogens isolated from the infectious turtles gave negative results, indicating that not all pathogenic A. hydrophlla and bacterial pathogens contain aer gene coding aerolysin. |
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| Keywords: | Aeromonas hydrophlla aer gene PCR |
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