| 木质素高效降解菌血红密孔菌产酶条件研究（英文） |
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| 作者单位： | WANG Dan-mei，REN Da-ming，LI Xiu-na，SHI Jiao College of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110161 |
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| 摘 要： | Objective] The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.Method] A strain of lignin-degrading white-rot fungus was selected from 5 strains of collected fungi and its ligninase production and the optimum conditions for producing ligninolytic enzyme were measured.Result]It could produce two kinds of ligninase with good thermal stability. Different temperatures, carbon sources, nitrogen sources,acidities, as well as the additions of surfactant had distinct influence on the development of lignin-degrading enzymes of the fungus. The optimum condition was drawn out: 38 ℃, pH=4.5, 10.0 g/L glucose, 1.0 g/L tartaric acid ammonium.Conclusion] The aim of research was to provide a basis for lignin degradation in practical production.
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Study on Fermentation Conditions of Pycnoporus sanguineus |
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| Authors: | WANG Dan-mei REN Da-ming LI Xiu-na SHI Jiao College of Biological Science and Technology Shenyang Agricultural University Shenyang |
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| Institution: | WANG Dan-mei,REN Da-ming,LI Xiu-na,SHI Jiao College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161 |
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| Abstract: | Objective] The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.Method] A strain of lignin-degrading white-rot fungus was selected from 5 strains of collected fungi and its ligninase production and the optimum conditions for producing ligninolytic enzyme were measured.Result]It could produce two kinds of ligninase with good thermal stability. Different temperatures, carbon sources, nitrogen sources,acidities, as well as the additions of surfactant had distinct influence on the development of lignin-degrading enzymes of the fungus. The optimum condition was drawn out: 38 ℃, pH=4.5, 10.0 g/L glucose, 1.0 g/L tartaric acid ammonium.Conclusion] The aim of research was to provide a basis for lignin degradation in practical production. |
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| Keywords: | White-rot fungus Lignin degradation Fermentation condition Orthogonal test |
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