| 两用核不育系水稻叶绿体DNA的比较研究 |
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| 引用本文： | 欧立军,李金龙,童梁,梁云浩,袁雅威.两用核不育系水稻叶绿体DNA的比较研究[J].中国农学通报,2010,26(11):50-53. |
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| 作者姓名： | 欧立军 李金龙 童梁 梁云浩 袁雅威 |
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| 作者单位： | 1. 民族药用植物资源研究与利用湖南省重点实验室,湖南怀化,418008;湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化,418008;怀化学院生命科学系,湖南怀化,418008
2. 怀化学院生命科学系,湖南怀化,418008 |
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| 基金项目： | 湖南省高校创新平台开放基金项目 |
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| 摘 要： | 以典型籼稻和粳稻作对照，对湖南应用较广的几个两用核不育系进行叶绿体DNA的ORF100、ORF29-TrnCGCA片段长度以及rps16基因内含子和TrnTUGU-TrnLUAA转录间区2个片段序列进行比较研究。结果表明，株1S、陆18S、株77S、H628S、88S、白天鹭S、G0543S、G03S、1103S和W6154S的叶绿体DNA的ORF100、ORF29-TrnCGCA两片段的大小均与典型的籼稻一致，其他材料则与典型粳稻相同；叶绿体DNA碱基多态性较丰富的2个区域rps16基因内含子和TrnT UGU -TrnL UAA间区的测序后发现4个籼粳稻特异位点，这4个位点对两用不育系叶绿体籼粳属性的判断与ORF100、ORF29-TrnCGCA片段的结果一致。根据育出组合的数量和质量，带有粳型血缘细胞质的两用核不育系如准S、810S、、中心S、C185S和培矮64S等显现了一定的优势。研究结果说明，通过籼粳稻杂交选育具粳稻血缘的籼型不育系是提高杂交水稻产量的有效途径之一。
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| 关 键 词： | 尖孢镰刀菌 尖孢镰刀菌 瓜类 致病性 β-D-葡萄糖苷酶 |
| 收稿时间： | 2010-01-07 |
| 修稿时间： | 2010-02-19 |
Chloroplast DNA polymorphism of dual purpose genic male sterile lines |
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| Ou Lijun,Li Jinlong,Tong Liang,Liang Yunhao,Yuan Yawei.Chloroplast DNA polymorphism of dual purpose genic male sterile lines[J].Chinese Agricultural Science Bulletin,2010,26(11):50-53. |
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| Authors: | Ou Lijun Li Jinlong Tong Liang Liang Yunhao Yuan Yawei |
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| Institution: | 1Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Huaihua Hunan 418008;
2Key Laboratory of Hunan Higher Education for Hunan-western Medicinal Plant and Ethnobotany, Huaihua Hunan 418008;
3Department of Life Sciences, Huaihua University, Huaihua Hunan 418008 |
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| Abstract: | The lengths of open reading frame (ORF)100 and ORF29-TrnCGCA, the intronic sequence of rps16 and the transcribed spacer of TrnTUGU-TrnLUAA in chloroplast of dual purpose genic male sterile line rice are studied using indica types and japonica types as controls. The results show that the lengths of ORF100 and ORF29-TrnCGCA in Zhu 1S, Lu 18S, Zhu 77S, H628S, 88S, Baitianlu S, G0543S, G03S, 1103S and W6154S are similar to those of typical indica, the others are similar to those of typical japonica. There are four special indica/japonica markers and the type of all dual purpose genic male sterile line rice based these special indica/japonica markers are same with the differentiation by ORF100 and ORF29-TrnCGCA. The materials such Zhun S, 810S, Zhongxin S, C185S and Peiai 64S those have japonica-type blood have more superiority than others based their quantity and quality of combination. Therefore, using dual purpose genic male sterile lines with japonica -type blood is an effective way to improve production of hybrid rice. |
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| Keywords: | japonica-type bloodzz |
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