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Gaseous nitrogen losses from fertilized soil during nitrification and denitrification using the acetylene blockage technique
Authors:S Casella  C Leporini  G Picci
Institution:(1) Centro di Studio per la Microbiologia del Suolo, CNR, Pisa, Instituto Microbiologia agraria, Università di Pisa, Via del Borghetto, 80, I-56100 Pisa, Italy
Abstract:Summary A sandy soil amended with different forms and amounts of fertilizer nitrogen (urea, ammonium sulphate and potassium nitrate) was investigated in model experiments for N2O emission, which may be evolved during both oxidation of ammonia to nitrate and anaerobic respiration of nitrate. Since C2H2 inhibits both nitrification and the reduction of N2O to N2 during denitrification, the amount of N2O evolved in the presence and absence of C2H2 represents the nitrogen released through nitrification and denitrification.Results show that amounts of N2O-N lost from soils incubated anaerobically with 0.1% C2H2 and treated with potassium nitrate (23.1 µg N-NO 3 /g dry soil) exceeded those from soils incubated in the presence of 20% oxygen and treated with even larger amounts of nitrogen as urea and ammonium sulphate. This indicates that nitrogen losses by denitrification may potentially be higher than those occurring through nitrification.
Keywords:Fertilized soil  Nitrification  Denitrification  N2O production  C2H2 blockage
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