| 大豆对斜纹夜蛾抗生性基因的微卫星标记(SSR)的研究 |
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| 引用本文： | 王慧,喻德跃,吴巧娟,盖钧镒.大豆对斜纹夜蛾抗生性基因的微卫星标记(SSR)的研究[J].大豆科学,2004,23(2):91-95. |
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| 作者姓名： | 王慧 喻德跃 吴巧娟 盖钧镒 |
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| 作者单位： | 南京农业大学大豆研究所,国家大豆改良中心,作物遗传与种质创新国家重点实验室,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家高技术研究发展计划(863计划) |
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| 摘 要： | 以皖82-178(抗)×通山薄皮黄豆甲(感)组合衍生的重组近交系群体为材料,以幼虫重为抗性鉴定指标,对大豆抗斜纹夜蛾的基因(QTL)进行SSR分子标记研究.从大豆公共连锁图谱(Cregan et al.,1999)7个与抗虫性可能有关的连锁群上选取了103对SSR引物,鉴定亲本和RIL家系DNA,其中18对引物在RIL家系间表现出多态性.应用t测验和方差分析法分析SSR标记与性状的连锁,并以R2表示标记对性状变异的解释率.结果在D2、C2、H连锁群上分别找到一个与大豆对斜纹夜蛾抗性有关的SSR标记,即Satt135、Satt363、Satt442,单个标记对抗性变异的解释率分别为5.57%、2.89%、8.7%.
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| 关 键 词： | 大豆(Glycine max(L.)MerrilD 斜纹夜蛾(Prodenia litura(L.)Fabricius) 抗生性 |
| 文章编号： | 1000-9841(2004)02-0091-05 |
CHARACTENRIZATION OF RESISTANCE GENES TO COTTON WORM WITH SSR MARKERS IN SOYBEAN |
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| Wang Hui Yu Deyue,Wu Qiaojuan Gai Junyi.CHARACTENRIZATION OF RESISTANCE GENES TO COTTON WORM WITH SSR MARKERS IN SOYBEAN[J].Soybean Science,2004,23(2):91-95. |
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| Authors: | Wang Hui Yu Deyue Wu Qiaojuan Gai Junyi |
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| Institution: | Wang Hui Yu Deyue * Wu Qiaojuan Gai Junyi |
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| Abstract: | There have been few reports about molecular marker analysis of resistance to insect in soybean. This study was to determine quantitative trait loci (QTLs) associated with the soybean resistance to insect (Cotton Worm) by estimating linkage to simple sequence repeat (SSR) markers. Recombinant inbred lines (RILs) constructed through a cross between Wan82-178 and Tongshanbopihuangdoujia was used. Larval weight was examined in as index of resistance. Using the intergrated genetic linkage map of soybean (Cregan et al. 1999), we chose 103 SSR markers scattering on 7 linkage groups (LGs) that had the potential to map resistant QTL to screen polymorphic markers between Wan82-178 and Tongshanbopihuangdoujia. It was found that 18 SSR primers showed polymorphic DNA bands among these RIL lines. With t-test analysis and analysis of variance, three markers (Satt135,Satt363,Satt434) were found linked with the resistance genes, situating on three LGs, i.e. D2, C2, and H. The individual effect of each marker was reported as R 2 value, i.e 5.57%, 2.89%, and 8.7% respectively. |
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| Keywords: | SSR |
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